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Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure. [PDF]

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Shim AR   +13 more
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In Situ Hybridization

Ultrastructural Pathology, 1989
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
Jahn M. Nesland   +1 more
openaire   +5 more sources

In situ Hybridization

2008
Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or ...
Tim D. Hewitson, Ian A. Darby
openaire   +4 more sources

In situ hybridization

Clinics in Dermatology, 1991
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
openaire   +5 more sources

In situ Hybridization

2013
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Scott A. Harding, Chung-Jui Tsai
openaire   +3 more sources

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