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Clinics in Laboratory Medicine, 1990
In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
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In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
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2001
Afascinating property of DNA is the complementarity of the nucleotide bases in its two anti-parallel strands, with G always pairing with C and A always pairing with T. This does not involve strong covalent chemical bonds but weak hydrogen bonds. There are three hydrogen bonds between G-C pairs and two between A-T pairs, so strand separation is easier ...
Eeva Therman, Orlando J. Miller
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Afascinating property of DNA is the complementarity of the nucleotide bases in its two anti-parallel strands, with G always pairing with C and A always pairing with T. This does not involve strong covalent chemical bonds but weak hydrogen bonds. There are three hydrogen bonds between G-C pairs and two between A-T pairs, so strand separation is easier ...
Eeva Therman, Orlando J. Miller
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1995
Publisher Summary This chapter focuses on the use of in situ hybridization for tissue sections with particular emphasis on paraffin-embedded material. Identification of the temporal and spatial patterns of gene expression can provide important clues about gene function. This holds particularly true in embryonic development.
Luis F. Parada, Lino Tessarollo
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Publisher Summary This chapter focuses on the use of in situ hybridization for tissue sections with particular emphasis on paraffin-embedded material. Identification of the temporal and spatial patterns of gene expression can provide important clues about gene function. This holds particularly true in embryonic development.
Luis F. Parada, Lino Tessarollo
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Measurement of In Situ Hybridization
Current Protocols in Molecular Biology, 2000AbstractHybridization of labeled specific molecular probes to nucleic acids in tissues allows geometric and functional location of gene expression or of foreign genome sequences. Estimates of amounts and location of target nucleic acid sequence can be made with phosphor storage imaging and molecular controls.
Michele Cottler-Fox +4 more
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2009
Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe.
Godavarthi B.K.S. Prasad +2 more
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Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe.
Godavarthi B.K.S. Prasad +2 more
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Metallographic in situ hybridization
Human Pathology, 2007Metallographic methods, in which a target is visualized using a probe or antibody that deposits metal selectively at its binding site, offers many advantages for bright-field in situ hybridization (ISH) detection as well as for other labeling and detection methods.
James F. Hainfeld +8 more
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Fluorescent In Situ Hybridization
2003Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.
Elaine K. Green, Sara A. Dyer
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Ultrastructure of in Situ Hybridization [PDF]
Ultrastructural Pathology, 1992 Techniques for the ultrastructural localization of structures identified by in situ hybridization are being developed for both preembedding labeling and labeling on thin sections (postembedding). Successful labeling of both RNA and DNA sequences has been reported in recent years.
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In Situ Hybridization Histochemistry [PDF]
Current Protocols in Toxicology, 2000 AbstractThis unit describes two methods of in situ hybridization: one uses an 35S‐labeled oligonucleotide probe and the other uses a digoxigenin‐labeled oligonucleotide probe on frozen, cryostat‐sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA.
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Chapter 12 In Situ Hybridization
1995Publisher Summary In situ hybridization is a cytochemical technique for localizing specific DNA or RNA sequences within an organism. Applications of in situ hybridization technology are diverse. The most common usage in plant cell biology is localization of a messenger RNA (mRNA) at the light microscopic level.
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