Results 211 to 220 of about 470,888 (230)
Some of the next articles are maybe not open access.
Fluorescence In Situ Hybridization
Clinics in Laboratory Medicine, 2011This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
openaire +2 more sources
1994
In situ hybridization techniques enable gene expression to be monitored in individual cells (Figure 18.1). Methods for use with the light microscope are as sensitive as northern blot analysis for detecting mRNAs that accumulate equally in all cells, and more sensitive than northern analysis for detecting mRNAs that are present in only a small ...
openaire +2 more sources
In situ hybridization techniques enable gene expression to be monitored in individual cells (Figure 18.1). Methods for use with the light microscope are as sensitive as northern blot analysis for detecting mRNAs that accumulate equally in all cells, and more sensitive than northern analysis for detecting mRNAs that are present in only a small ...
openaire +2 more sources
1993
Hybridization of a nucleic acid probe to a nucleic acid target within cytological preparations permits a high degree of resolution as to the spatial localization of this target sequence. This localization can be useful in a number of ways for answering biological questions.
Jan A. Bayer, Ger van den Engh
openaire +2 more sources
Hybridization of a nucleic acid probe to a nucleic acid target within cytological preparations permits a high degree of resolution as to the spatial localization of this target sequence. This localization can be useful in a number of ways for answering biological questions.
Jan A. Bayer, Ger van den Engh
openaire +2 more sources
The Technique of In Situ Hybridization
2003In situ hybridization (ISH) (also called "hybridization histochemistry" or "hybridization cytology") was first described in 1969 by Gall and Pardue who used the technique to localize ribosomal DNA in Xenopus oocytes (1). In contrast to other techniques of hybridization (i.e., Northern or Southern blotting), ISH allows detection of specific DNA or RNA ...
Sun Ying, A. Barry Kay
openaire +3 more sources
Applications of in Situ Hybridization
1991Publisher Summary This chapter discusses applications of in situ hybridization(ISH). Progress in molecular biology has made several techniques for research and diagnosis available even to laboratories not specialized in this field. The basis of all these techniques is the ability of single-stranded nucleic acids, either deoxyribonucleic acid or ...
Hermann Herbst, Gerald Niedobitek
openaire +3 more sources
Fluorescence in situ hybridization
Blood Reviews, 1993Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
openaire +2 more sources
MicroRNA In Situ Hybridization
2011In situ hybridization (ISH) is a technology that allows detection of specific nucleic acid sequences in tissue samples at the cellular level. For detection of individual microRNAs (miRNAs) and mRNAs, the ISH technology determines the cellular origin of expression and provides information on expression levels in different tissue compartments and cell ...
openaire +3 more sources
, 2004