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Image analysis for bright-field HER2 in situ hybridization: validation for clinical use. [PDF]
Virchows ArchShi R +5 more
europepmc +1 more source
High-throughput single telomere analysis using DNA microarray and fluorescent in situ hybridization. [PDF]
Nucleic Acids ResZheng YL +7 more
europepmc +1 more source
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2003
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+12 more sources
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+12 more sources
Ultrastructural Pathology, 1989
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
+6 more sources
Twenty years ago Gall and Pardue1 reported a method to detect RNA-DNA hybrid molecules in cytologic specimens. Since then diagnostic in situ hybridization has become a standard method to detect DNA and RNA.The method has also been modified for use in electron microscopy2-4 but it is not easy to preserve both the hybridization properties and an ...
J M, Nesland, J V, Johannessen
+6 more sources
Clinics in Dermatology, 1991
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources
Abstract Although several molecular biology techniques can be used to measure mRNA, only in situ hybridization (or in situ transcription) permit specific localization of DNA or an mRNA species within a tissue section or cell preparation. With appropriate fixation, mRNAs can be preserved and detected in tissue sections by using DNA or RNA probes ...
+6 more sources
2001
Abstract Gall and Pardue (1969) and John et al. (1969) were the first to localize nucleic acids directly to biological material (1, 2). They used radioactively labelled RNA to detect ribosomal DNA (rDNA) in cytological preparations. Since then, and particularly since the early 1990s, the uses of the method have exploded, so much so ...
A R Leitch, K Y Lim
openaire +2 more sources
Abstract Gall and Pardue (1969) and John et al. (1969) were the first to localize nucleic acids directly to biological material (1, 2). They used radioactively labelled RNA to detect ribosomal DNA (rDNA) in cytological preparations. Since then, and particularly since the early 1990s, the uses of the method have exploded, so much so ...
A R Leitch, K Y Lim
openaire +2 more sources
2013
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
Current Protocols in Neuroscience, 2019
Fluorescent detection of transcripts using RNAscope has quickly become a standard in situ hybridization (ISH) approach in neuroscience with over 400 publications since its introduction in 2012.
L. Erben, A. Buonanno
semanticscholar +1 more source
Fluorescent detection of transcripts using RNAscope has quickly become a standard in situ hybridization (ISH) approach in neuroscience with over 400 publications since its introduction in 2012.
L. Erben, A. Buonanno
semanticscholar +1 more source