Results 321 to 330 of about 617,973 (397)
Some of the next articles are maybe not open access.
Production of Digoxigenin‐Labeled Riboprobes for In Situ Hybridization Experiments
Current Protocols in Mouse Biology, 2020Experiments that visualize gene expression in intact tissues or organisms are fundamental to studies of gene function. These experiments, called in situ hybridization, require the production of a riboprobe, which is a labeled antisense RNA corresponding ...
Kristen S Barratt, R. Arkell
semanticscholar +1 more source
Fluorescence In Situ Hybridization
2003In situ hybridization describes the annealing of a labeled nucleic acid to complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the location of the probe.
Barbara A, Tate, Rachel L, Ostroff
openaire +4 more sources
Metallographic in situ hybridization
Human Pathology, 2007Metallographic methods, in which a target is visualized using a probe or antibody that deposits metal selectively at its binding site, offers many advantages for bright-field in situ hybridization (ISH) detection as well as for other labeling and detection methods.
Richard D, Powell +6 more
openaire +2 more sources
Seminars in diagnostic pathology, 2019
In situ hybridization (ISH) has become a common laboratory technique used for the analysis of gene expression and for the localization of specific DNA and RNA molecules in cells. Many different methods of performing ISH have been described.
Y. Chu +4 more
semanticscholar +1 more source
In situ hybridization (ISH) has become a common laboratory technique used for the analysis of gene expression and for the localization of specific DNA and RNA molecules in cells. Many different methods of performing ISH have been described.
Y. Chu +4 more
semanticscholar +1 more source
Fluorescent In Situ Hybridization
2003Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.
Sara A, Dyer, Elaine K, Green
openaire +2 more sources
Fluorescence In Situ Hybridization
Clinics in Laboratory Medicine, 2011This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
openaire +2 more sources
2008
Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or ...
openaire +2 more sources
Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or ...
openaire +2 more sources
In Situ Hybridization Histochemistry
Current Protocols in Toxicology, 2000AbstractThis unit describes two methods of in situ hybridization: one uses an 35S‐labeled oligonucleotide probe and the other uses a digoxigenin‐labeled oligonucleotide probe on frozen, cryostat‐sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA.
openaire +2 more sources
Fluorescence in situ hybridization
Blood Reviews, 1993Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
openaire +2 more sources