The Journal of Reproduction and Development, 2022 We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval.
Takumi YOSHIDA +8 more
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An Extended Culture System that Supports Human Primordial Germ Cell-like Cell Survival and Initiation of DNA Methylation Erasure. [PDF]
, 2020 The development of an in vitro system in which human primordial germ cell-like cells (hPGCLCs) are generated from human pluripotent stem cells (hPSCs) has been invaluable to further our understanding of human primordial germ cell (hPGC) specification ...
Bower, Grace +9 more
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Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]
, 2020 The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Aldave, Anthony J +14 more
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In vitro production of bovine embryos derived from individual donors in the Corral® dish [PDF]
, 2017 Background: Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor.
A Cebrian-Serrano +38 more
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GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays
Applied SciencesThe temporary immersion system bioreactor named GreenTray® presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, nor is the use of a scalpel necessary to divide ...
Ramon Dolcet-Sanjuan +8 more
doaj +1 more source
In Vitro Porcine Colon Culture
, 2018 Models have been extensively used to investigate disease pathogenesis. Animal models are costly, require extensive logistics for animal care, and samples are not always suitable for different analytical techniques or to answer the research question. In vitro cell culture models are generally focused on recreating a specific characteristic of an organ ...
Costa, Matheus O. +3 more
openaire +4 more sources
Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus spp. Embryos
PlantsThe major factors affecting the in vitro immature embryo rescue efficiencies from Prunus persica or P. armeniaca accessions have been identified, along with improving the feasibility.
Maria Casanovas +2 more
doaj +1 more source
An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]
, 2015 The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael +2 more
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Initial in vitro Establishment of the Native Cerrado Orchid Miltonia Flavescens
Floresta e Ambiente, 2020 The situation of native orchids from natural environments becomes more vulnerable each day, especially those not protected by conservation units. Thus, studies on the propagation of species, especially on in vitro germination and initial establishment ...
Camila Soares Rosa Lemes +4 more
doaj +1 more source
Echinococcus, Giardia and Cryptosporidium: observational studies challenging accepted dogma [PDF]
, 2009 The development of in vitro culture systems that allow the maintenance, and support the development of Echinococcus, Giardia and Cryptosporidium in the laboratory have had a significant impact on their biology and taxonomy and the epidemiology of ...
Thompson, R.C.A.
core +2 more sources