Results 21 to 30 of about 14,911 (263)
IN VITRO DEGRADATION OF ATRACURIUM IN HUMAN PLASMA
British Journal of Anaesthesia, 1985 The degradation of atracurium and the formation of laudanosine was examined in vitro in both Sorensen buffer and human plasma using sensitive, specific high pressure liquid chromatographic assays to determine drug concentrations. At normal physiological pH and temperature, the degradation of atracurium was threefold more rapid in plasma than in buffer.
R L, Stiller, D R, Cook, S, Chakravorti
openaire +2 more sources
In vitro ruminal degradability of cereal grain starch
Czech Journal of Animal Science, 2004 An in vitro method was used to determine ruminal degradability of starch (IVRDS) in a set of cereal grains. The set included 9 feed samples, including 2 samples of ground wheat, 2 samples of wheat treated with sodium hydroxide, ground barley, barley ...
O. Tománková, P. Homolka
doaj +1 more source
Protein degradability of grassland forage under simulated rotational spring grazing [PDF]
Journal of Agricultural Sciences (Belgrade), 2019 A cutting experiment was conducted to analyze the changes in the crude protein (CP) fraction content and in the estimated ruminal protein degradability of forage, obtained in conditions of simulated rotational spring grazing on permanent grassland.
Stojanović Bojan D. +5 more
doaj
IN VITRO DEGRADATION OF ATRACURIUM IN HUMAN PLASMA
British Journal of Anaesthesia, 1983 Degradation of the neuromuscular blocking agent atracurium has been examined in vitro in both Tris buffer and human plasma using a biological assessment in the mouse to estimate active drug concentrations. These studies show that at normal physiological pH and temperature, the major route of inactivation is by “Hofmann elimination”.
R A, Merrett, C W, Thompson, F W, Webb
openaire +2 more sources
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
In vitro and in vivo degradation correlations for polyurethane foams with tunable degradation rates
Journal of Biomedical Materials Research Part A, 2023 AbstractPolyurethane foams present a tunable biomaterial platform with potential for use in a range of regenerative medicine applications. Achieving a balance between scaffold degradation rates and tissue ingrowth is vital for successful wound healing, and significant in vivo testing is required to understand these processes.
Anand Utpal Vakil +9 more
openaire +2 more sources
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source