Results 321 to 330 of about 670,418 (378)

PRELIVE: A Framework for Predicting Lipid Nanoparticles In Vivo Efficacy and Reducing Reliance on Animal Testing

open access: yesAdvanced Functional Materials, EarlyView.
PREdicting LNP In Vivo Efficacy (PRELIVE) framework enables the prediction of lipid nanoparticle (LNPs) organ‐specific delivery through dual modeling approaches. Composition‐based models using formulation parameters and protein corona‐based models using biological fingerprints both achieve high predictive accuracy across multiple organs.
Belal I. Hanafy   +3 more
wiley   +1 more source

Dual‐Layer Living Hydrogel Enables On‐Demand Delivery of Phages and Probiotics for Synergistic Wound Infection Therapy

open access: yesAdvanced Functional Materials, EarlyView.
A dual‐layer living hydrogel, ProΦGel, integrates bacteriophages and probiotics for synergistic wound infection therapy. The outer gelatin‐based matrix releases phages on demand in response to P. aeruginosa infections, while inner alginate beads sustain probiotic delivery.
Siyuan Tao   +6 more
wiley   +1 more source

Nanodiamond‐Based Quantum Sensing Reveals Changes in Endo‐Lysosomal Activity of Stimulated Cardiac Fibroblasts in a Cellular Fibrosis Model

open access: yesAdvanced Functional Materials, EarlyView.
Quantum sensing reveals intricate patterns linking endo‐lysosomal maturation to cardiac fibrosis progression, highlighting complexity in cellular remodeling. This study investigates fibroblast‐to‐myofibroblast transition under cell aging, stiffness, and TGF‐β stimulation, comparing nanodiamond uptake, endo‐lysosomal dynamics, and free radical ...
Aldona Mzyk   +3 more
wiley   +1 more source

In Vitro Expansion of Corneal Endothelial Cells for Transplantation.

Methods in molecular biology, 2020
The corneal endothelium forms a leaky barrier between the corneal stroma and the aqueous humor of the anterior chamber. This cell monolayer maintains the corneal stroma in a state of relative dehydration, a process called deturgescence, which is required in order to obtain corneal stromal transparency. Endothelial dysfunctions lead to visual impairment
Kim Santerre   +3 more
semanticscholar   +3 more sources

In vitro expansion of Vγ9Vδ2 T cells for immunotherapy.

Methods in Enzymology, 2020
Vγ9Vδ2 T cells can exhibit potent anti-tumor activity and have gained much attention as an interesting tool for cancer immunotherapy by adoptive cell transfer. In this chapter, we explain the different approaches for the in vitro expansion of Vγ9Vδ2 T cells for their subsequent adoptive transfer and present a detailed protocol for the successful in ...
C. Peters   +4 more
semanticscholar   +3 more sources

Isolation and in vitro expansion of porcine spermatogonial stem cells.

Reproduction in domestic animals = Zuchthygiene, 2021
Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genetic information to offspring through their ability to both self-renew and differentiate into mature spermatozoa.
Xin Zhao   +6 more
semanticscholar   +1 more source

In vitro expansion of hematopoietic stem cells

Stem Cells, 1997
We have investigated the effects of interleukin 3 (IL-3) and IL-1 on in vitro expansion of murine hematopoietic progenitors and stem cells using a highly purified progenitor population. Lineage negative, Ly-6A/E+, c-kit+ bone marrow cells from male mice were cultured in suspension in the presence of stem cell factor, IL-6, IL-11 and erythropoietin with
M, Ogawa, Y, Yonemura, H, Ku
openaire   +2 more sources

In vitro expansion patterns of ameroid ring constrictors

American Journal of Veterinary Research, 2008
Abstract Objective—To evaluate and quantify in vitro dimensional changes of ameroid ring constrictors (ARCs) with and without the outer stainless steel ring in place over time. Sample Population—12 ARCs (5.0-mm diameter). Procedures—6 ARCs were immersed in canine plasma baths for 34 days without the stainless steel outer ring in place (group N), and 6 ...
Steven S, Leshem   +2 more
openaire   +2 more sources

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