Results 211 to 220 of about 392,164 (310)

Morphological and Functional Evaluation of Kodkod (<i>Leopardus guigna</i>) Oocytes After In Vitro Maturation and Parthenogenetic Activation. [PDF]

Animals (Basel)
Toledo-Saldivia D, Cáceres-Hernández A, Doussang D, Zapata-Rojas C, Vergara S, Carvacho I, Castro FO, Rodriguez-Alvarez L, Veraguas-Dávila D.   +8 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

EFNA5 as an Oocyte-Derived Factor Enhances Developmental Competence by Modulating Oxidative Stress, Inflammation, and Apoptosis During In Vitro Maturation. [PDF]

Antioxidants (Basel)
Liu X, Cui J, Wang Y, Hao J, Wu Y, Wang Y, An L, Tian J, Xi G.   +8 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes. [PDF]

Mol Reprod Dev
Cadenas J, Adrados CS, Kristensen SG, Macklon KT, Greve VH, Fedder J, Mamsen LS, Andersen CY.   +7 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Effects of physiological oxygen tension on human cumulus-oocyte-complex metabolism during in vitro maturation: an exploratory study. [PDF]

J Ovarian Res
Anckaert E, Ates G, Liveyns A, Van Ranst H, Mostinckx L, Cools W, Mortier A, Massie A, De Vos M, Akin N.   +9 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Cumulative live birth after unstimulated in vitro maturation versus conventional in vitro fertilization (IVF) in women with polycystic ovary syndrome: a 36-month retrospective matched cohort study. [PDF]

J Ovarian Res
Guo W, Chen L, Yang Z, Wang L, Wu H, Yang R, Yang S, Zheng X, Chang HM, Zhen X, Qiao J, Li R.   +11 more
europepmc   +1 more source