Enhancing oocyte in vitro maturation and quality by melatonin/bilirubin cationic nanoparticles: A promising strategy for assisted reproduction techniques [PDF]
International Journal of Pharmaceutics: XIn assisted reproduction techniques, oocytes encounter elevated levels of reactive oxygen species (ROS) during in vitro maturation (IVM). Oxidative stress adversely affects oocyte quality, hampering their maturation, growth, and subsequent development ...
Haitao Xi +12 more
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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system. [PDF]
PLoS ONE, 2023 Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and ...
Giuliana de Avila Ferronato +7 more
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Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat [PDF]
Czech Journal of Animal Science, 2007 The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were counted, respectively. The good-quality oocytes (good
Z.G. Wang, Z.R. XU, S.D. YU
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The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by in vitro maturation [PDF]
Veterinary World, 2020 Background and Aim: The combination of vitrification techniques and in vitro maturation can reduce oocyte competence. Mitogen-activated protein kinase and maturation-promoting factor are significant in oocyte meiotic maturation regulation.
A. A. Muhammad Nur Kasman +2 more
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Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining [PDF]
Veterinary World, 2021 Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation ...
Mohamed Fathi, Amr F. Elkarmoty
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Frontiers in Veterinary Science, 2023 Porcine embryos are used for a variety of applications. However, the maturation rate in vitro remains low, and novel in vitro maturation (IVM) techniques that facilitate the collection of mature oocytes are necessary.
Sohee Kim +20 more
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Indian Journal of Animal Sciences, 2014 The study was conducted to know the effect of oocyte collection techniques and various maturation media on in vitro maturation of buffalo oocytes obtained from slaughterhouse derived ovaries.
C F CHAUDHARI +4 more
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In vitro techniques of bovine oocyte maturation, fertilization and embryo culture resulting in the birth of a calf [PDF]
Reproduction Nutrition Développement, 1989 Oocyte cumulus complexes were aspirated from 3 to 5 mm follicles of cows prestimulated with 2.000 IU PMSG 24 h before slaughter. Oocytes matured in culture were fertilized in vitro by heparinized freshly ejaculated or epididymal spermatozoa. The cultivation procedure for fertilized eggs was the same as that used for cultivation of oocytes.
Pavlok, A. +3 more
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How to collect immature oocytes from small antral follicles for the Saigon biphasic in vitro maturation protocol [PDF]
F&S ReportsObjective: To provide a detailed description of the clinical procedure for aspirating small antral follicles (2.0–8.0 mm) as part of the Saigon biphasic in vitro maturation (IVM) protocol.
Lan N. Vuong, M.D., Ph.D. +9 more
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The Indian Journal of Animal Sciences, 2016 The present study was conducted to evaluate the effectiveness of single-step and two-step vitrification techniques on post-thaw survivability and subsequent in vitro maturation of immature bovine oocytes. In two-step vitrification method, oocytes were first equilibrated in vitrification solution-I and then vitrification solution–II of 3.5M or 4.0M and ...
B SAIKIA +6 more
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