Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat [PDF]
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were counted, respectively. The good-quality oocytes (good
Z.G. Wang, Z.R. XU, S.D. YU
doaj +2 more sources
The study was conducted to know the effect of oocyte collection techniques and various maturation media on in vitro maturation of buffalo oocytes obtained from slaughterhouse derived ovaries.
C F CHAUDHARI +4 more
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In assisted reproduction techniques, oocytes encounter elevated levels of reactive oxygen species (ROS) during in vitro maturation (IVM). Oxidative stress adversely affects oocyte quality, hampering their maturation, growth, and subsequent development ...
Haitao Xi +12 more
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Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system [PDF]
Background To assess the impact of oil overlay during maturation of immature oocytes in vitro in our optimized liquid culture system. Methods We conducted comparative analyses of pH, osmolality, and the levels of melatonin, FSH, E2, and hCG in the ...
Jing Peng +4 more
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Clinical outcomes over 12 years: a comparative analysis of oocyte cryopreservation from controlled ovarian stimulation and in vitro maturation retrieved from ovarian tissue [PDF]
Background Fertility preservation is crucial for females facing gonadotoxic treatments. Controlled ovarian stimulation (COS) followed by oocyte cryopreservation is a well-established method, while ovarian tissue-derived oocyte in vitro maturation (OTO ...
Xiaoping Liu +8 more
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The present study was conducted to evaluate the effectiveness of single-step and two-step vitrification techniques on post-thaw survivability and subsequent in vitro maturation of immature bovine oocytes.
B SAIKIA +6 more
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The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by in vitro maturation [PDF]
Background and Aim: The combination of vitrification techniques and in vitro maturation can reduce oocyte competence. Mitogen-activated protein kinase and maturation-promoting factor are significant in oocyte meiotic maturation regulation.
A. A. Muhammad Nur Kasman +2 more
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Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining [PDF]
Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation ...
Mohamed Fathi, Amr F. Elkarmoty
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Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique [PDF]
AbstractTo investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date.
Lingsheng Chen +10 more
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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.
Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and ...
Giuliana de Avila Ferronato +7 more
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