International Journal of Pharmaceutics: XIn assisted reproduction techniques, oocytes encounter elevated levels of reactive oxygen species (ROS) during in vitro maturation (IVM). Oxidative stress adversely affects oocyte quality, hampering their maturation, growth, and subsequent development ...
Haitao Xi +12 more
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Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat [PDF]
Czech Journal of Animal Science, 2007 The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were counted, respectively. The good-quality oocytes (good
Z.G. Wang, Z.R. XU, S.D. YU
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Indian Journal of Animal Sciences, 2014 The study was conducted to know the effect of oocyte collection techniques and various maturation media on in vitro maturation of buffalo oocytes obtained from slaughterhouse derived ovaries.
C F CHAUDHARI +4 more
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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.
PLoS ONE, 2023 Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and ...
Giuliana de Avila Ferronato +7 more
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How to collect immature oocytes from small antral follicles for the Saigon biphasic in vitro maturation protocol [PDF]
F&S ReportsObjective: To provide a detailed description of the clinical procedure for aspirating small antral follicles (2.0–8.0 mm) as part of the Saigon biphasic in vitro maturation (IVM) protocol.
Lan N. Vuong, M.D., Ph.D. +9 more
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In vitro maturation (IVM) of camel oocytes using different maturation media [PDF]
Benha Veterinary Medical Journal, 2023 The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using different maturation media. Ovaries of 25 she-camel were collected during the breeding season (from December to April). Ovaries were transferred to the lab in
Shimaa Gwaid +3 more
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The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by in vitro maturation [PDF]
Veterinary World, 2020 Background and Aim: The combination of vitrification techniques and in vitro maturation can reduce oocyte competence. Mitogen-activated protein kinase and maturation-promoting factor are significant in oocyte meiotic maturation regulation.
A. A. Muhammad Nur Kasman +2 more
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Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining [PDF]
Veterinary World, 2021 Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation ...
Mohamed Fathi, Amr F. Elkarmoty
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A novel technique for in vitro maturation of sheep oocytes in a liquid marble microbioreactor [PDF]
Journal of Assisted Reproduction and Genetics, 2016 The aim of this work was to develop a microbioreactor using liquid marble (LM) as a novel system for oocyte in vitro maturation (IVM) in small volumes.Cumulus-oocyte complexes (COCs) obtained from slaughterhouse sheep ovaries were in vitro matured in a LM system prepared by placing a drop (30 μl containing 10 COCs) suspended in TCM 199 supplemented ...
Ledda S +5 more
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In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques [PDF]
Journal of Assisted Reproduction and Genetics, 2000 Our purpose was to investigate the factors influencing maturation and fertilization of immature oocytes.Immature oocytes were obtained from women undergoing cesarean section. They were cultured in the maturation medium either with or without cumulus cells.
J L, Hwang, Y H, Lin, Y L, Tsai
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