Results 41 to 50 of about 114,907,480 (246)

Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique

Turkish Journal of Veterinary & Animal Sciences, 2009
This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries.
Abdullah, Ramli, Wan-Khadijah, W.E., Rahman, A.   +2 more
openaire   +1 more source

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

Buletin Peternakan, 2020
This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1;  the storage duration of ovaries were 6, 9 and
Udin, Zaituni, Masrizal, Masrizal, Hendri, Hendri, Nanda, Syafri   +3 more
openaire   +3 more sources

Spatially Organized Human Ovarian Spheroids Instruct Endometrial Morphogenesis

Advanced Science, EarlyView.
A spatially organized human follicle‐like spheroid (SPHEGaT) composed of granulosa cells (GCs) and stromal‐derived theca‐like cells (TLCs) establishes sustained steroidogenesis in vitro. When co‐cultured with human endometrial organoids (EMOs), estradiol (E2) and progesterone (P4) exchange induces secretory/progesterone‐responsive differentiation ...
Maria João Sousa, Silke De Vriendt, Lixian Liu, Thalles Fernando Rocha Ruiz, Hugo Vankelecom, Christiani Andrade Amorim   +5 more
wiley   +1 more source

HLA-G MOLECULES: FROM EMBRYO IMPLANTATION TO OOCYTE MATURATION [PDF]

, 2009
Background Assisted reproduction technique (ART) pregnancy rates have not changed in recent years and an increased risk of twins, triplets or higher order pregnancies leads to a perinatal mortality and morbidity.
Rizzo, Roberta
core  

Effect of vitrification techniques on post-thaw survivability and in vitro maturation of immature bovine oocytes

The Indian Journal of Animal Sciences, 2016
The present study was conducted to evaluate the effectiveness of single-step and two-step vitrification techniques on post-thaw survivability and subsequent in vitro maturation of immature bovine oocytes. In two-step vitrification method, oocytes were first equilibrated in vitrification solution-I and then vitrification solution–II of 3.5M or 4.0M and ...
B SAIKIA, P M BARUA, D DUTTA, B C DEKA, M DUTTA CHOUDHURY, H DEV, S HANDIQUE   +6 more
openaire   +2 more sources

Obstetrical and Neonatal Outcomes of 3,028 Singletons Born After Advanced ART Techniques: Ejaculated Sperm Intracytoplasmic Sperm Injection, Artificial Oocyte Activation, in Vitro Maturation and Testicular Sperm Extraction

Fertility & Reproduction, 2021
Background: To evaluate obstetrical and neonatal outcomes of singletons conceived after advanced assisted reproductive technology (ART) techniques: conventional IVF pregnancies (C-IVF), ejaculated sperm intracytoplasmic sperm injection (ICSI), assisted oocyte activation (AOA), in vitro maturation (IVM), and testicular sperm extraction (TESE). Methods:
Hiromitsu Hattori, Yuko Atsumi, Yukiko Nakajo, Nobuya Aono, Masae Koizumi, Mayumi Toya, Hideki Igarashi, Koichi Kyono   +7 more
openaire   +2 more sources

Deciphering Anti‐Cancer Drug Efficacy Through Nanomechanical Vibrations in Living Gastric Cancer Organoids

Advanced Science, EarlyView.
This study developed an efficacy assessment platform that integrates patient‐derived gastric cancer organoids, atomic force microscopy (AFM)‐based nanomechanical vibration detection, deep learning analysis, and organoid mechanical modeling. It detects picomolar drug effects within 0.1 s signal, achieves 97% classification accuracy, and offers non ...
Ting Zhang, Qiubo Chen, Shihai Lan, Liang Zhu, Shuaiyu Liu, Yan Xia, Yalu Zhang, Hanhui Yao, Yongman Liu, Shangquan Wu, Qingchuan Zhang   +10 more
wiley   +1 more source

Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries [PDF]

Veterinary World, 2014
Aim: The present study was aimed at assessing the influence of three oocyte harvesting (aspiration, puncture and slicing) methods and presence or absence of corpus luteum (CL) on recovery efficiency and subsequent in vitro maturation and parthenogenetic ...
Yelisetti Uma Mahesh, Murakonda Mutha Rao, Poda Sudhakar, Krothapalli Raja Surya Sambasiva Rao   +3 more
doaj   +1 more source

SDF‐1 Attenuates Oocyte Quality Decline During Reproductive Aging Through Autophagy‐Enhanced Stress Granule Scavenging

Advanced Science, EarlyView.
SDF‐1 levels decline significantly with maternal aging. Exogenous supplementation restores meiotic spindle morphology, chromosomal alignment, and mitochondrial function while reducing oxidative stress in aged oocytes. Mechanistically, SDF‐1 enhances autophagic activity to clear accumulated stress granules, thereby rescuing fertilization competence and ...
Rui Long, Meng Wang, Ruolin Mao, Hong Chen, Juepu Zhou, Xiangfei Wang, Zheng Yuan, Ri‐Cheng Chian, Nan Xiao, Qingsong Xi, Yimin Shu, Lei Jin, Lixia Zhu   +12 more
wiley   +1 more source

Effect of cAMP on bovine oocyte maturation in vitro [PDF]

, 2021
Cyclic nucleotides cAMP and cGMP are among the main molecules that control the maturation of mammal oocytes. The in vitro simulated physiological oocyte maturation (SPOM) method was used to model cAMP accumulation in the oocyte.
Anna Krivonogova, Alexey Deykin, Albina Isaeva, Krivonogova Anna, Valeria Makutina, Isaeva Albina, Makutina Valeria, Deykin Alexey   +7 more
core   +2 more sources