Background: The three-parent assisted reproductive technique may increase oocyte competence.Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer ...
Sara Darbandi +10 more
doaj +1 more source
Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique [PDF]
AbstractTo investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date.
Lingsheng Chen +10 more
openaire +2 more sources
Collection of 125 Oocytes in an In Vitro Maturation Cycle Using a New Oocyte Collection Technique: A Case Report [PDF]
We have developed a new oocyte collection technique applicable to use in women with "string-of-pearls" polycystic ovaries undergoing in vitro maturation (IVM) of oocytes for in vitro fertilization.A 34-year-old woman with polycystic ovary syndrome and infertility underwent IVM.
Michael H, Dahan +5 more
openaire +2 more sources
2023 Acuerdos transformativos CRUEIn vitro maturation (IVM) system is an alternative method to superovulation protocols to obtain mature oocytes. Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) have been widely used in IVM medium ...
Cañón Beltrán, Karina Esperanza +5 more
core +1 more source
In vitro techniques of bovine oocyte maturation, fertilization and embryo culture resulting in the birth of a calf [PDF]
Oocyte cumulus complexes were aspirated from 3 to 5 mm follicles of cows prestimulated with 2.000 IU PMSG 24 h before slaughter. Oocytes matured in culture were fertilized in vitro by heparinized freshly ejaculated or epididymal spermatozoa. The cultivation procedure for fertilized eggs was the same as that used for cultivation of oocytes.
Pavlok, A. +3 more
openaire +3 more sources
The Role of Cumulus Cells for The Immature Oocyte Undergoing In Vitro Maturation Rescue: A Retrospective Analysis [PDF]
Background: Lack of matured oocytes after ovarian stimulation for in vitro fertilization (IVF) is one problem affecting the number of good-quality embryos.
Yudha Sudewo +8 more
doaj +1 more source
Role of Mitochondria Transfer in Infertility: A Commentary
Mitochondria transfer techniques were first designed to prevent the transmission of diseases due to mutations in mtDNA, as these organelles are exclusively transmitted to the offspring by the oocyte.
Cristina Rodríguez-Varela +1 more
doaj +1 more source
A physiological approach for supporting oocyte-cumulus cell communication and chromatin remodelling at meiotic resumption [PDF]
In vitro embryo production (IVP) remains inefficient in both clinical applications of human reproduction and animal breeding and the development of pre-maturation strategies to improve IVP is a mayor challenge in assisted reproductive technologies.
A.M. Luciano
core +2 more sources
Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development. [PDF]
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception.
Juliano C da Silveira +14 more
doaj +1 more source
Current Relevant Knowledge on Dog Reproductive Physiology – a Review
Successful in vitro techniques depend on the possibility to mimic in vivo conditions. For this reason, knowledge on reproductive physiology is necessary to define an efficient entirely in vitro system to produce embryos.
Ioana Hașegan +5 more
doaj

