Cryopreservation of equine oocytes: looking into the crystal ball [PDF]
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel +4 more
core +2 more sources
In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques [PDF]
Our purpose was to investigate the factors influencing maturation and fertilization of immature oocytes.Immature oocytes were obtained from women undergoing cesarean section. They were cultured in the maturation medium either with or without cumulus cells.
J L, Hwang, Y H, Lin, Y L, Tsai
openaire +2 more sources
Background: The three-parent assisted reproductive technique may increase oocyte competence.Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer ...
Sara Darbandi +10 more
doaj +1 more source
A novel technique for in vitro maturation of sheep oocytes in a liquid marble microbioreactor [PDF]
The aim of this work was to develop a microbioreactor using liquid marble (LM) as a novel system for oocyte in vitro maturation (IVM) in small volumes.Cumulus-oocyte complexes (COCs) obtained from slaughterhouse sheep ovaries were in vitro matured in a LM system prepared by placing a drop (30 μl containing 10 COCs) suspended in TCM 199 supplemented ...
Ledda S +5 more
openaire +3 more sources
Collection of 125 Oocytes in an In Vitro Maturation Cycle Using a New Oocyte Collection Technique: A Case Report [PDF]
We have developed a new oocyte collection technique applicable to use in women with "string-of-pearls" polycystic ovaries undergoing in vitro maturation (IVM) of oocytes for in vitro fertilization.A 34-year-old woman with polycystic ovary syndrome and infertility underwent IVM.
Michael H, Dahan +5 more
openaire +2 more sources
Localisation of RNAs into the germ plasm of vitellogenic xenopus oocytes [PDF]
We have studied the localisation of mRNAs in full-grown Xenopus laevis oocytes by injecting fluorescent RNAs, followed by confocal microscopy of the oocyte cortex.
B Heinrich +66 more
core +6 more sources
The Role of Cumulus Cells for The Immature Oocyte Undergoing In Vitro Maturation Rescue: A Retrospective Analysis [PDF]
Background: Lack of matured oocytes after ovarian stimulation for in vitro fertilization (IVF) is one problem affecting the number of good-quality embryos.
Yudha Sudewo +8 more
doaj +1 more source
Porcine oocyte maturation in vitro : role of cAMP and oocyte-secreted factors: a practical approach [PDF]
Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop and their quality is inferior to that of in ...
Appeltant, Ruth +4 more
core +2 more sources
Role of Mitochondria Transfer in Infertility: A Commentary
Mitochondria transfer techniques were first designed to prevent the transmission of diseases due to mutations in mtDNA, as these organelles are exclusively transmitted to the offspring by the oocyte.
Cristina Rodríguez-Varela +1 more
doaj +1 more source
Mitochondria directly influence fertilisation outcome in the pig [PDF]
The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome.
Abeydeera +66 more
core +3 more sources

