Results 181 to 190 of about 118,389,530 (261)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Shear Difference: Flow Type Dictates Endothelial Flow-Responsive Gene Programs in a 3-Dimensional-Printed In Vitro Model. [PDF]

J Am Heart Assoc
Shah NA, Rye KA, Endre ZH, Barber TJ, Erlich JH, Cochran BJ.   +5 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Wild blueberry-derived polyphenol metabolites attenuate telomere shortening in an in vitro model of metabolic syndrome. [PDF]

Eur J Nutr
Rendine M, Del Bo' C, Riso P, Møller P.
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

In Vitro Model of Milking Pulses and Stretching Cycles: Assessing Mechanical Effects on Bovine Mammary Cells. [PDF]

J Mammary Gland Biol Neoplasia
Dabby G, German Y, Toledano LN, Zaharoni N, Bar-Shira E, Belausov E, Brill-Karniely Y.   +6 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

(In Vitro Cellular&Developmental Biology Plant,35:061-069)A protocol for efficient transformation and regeneration of Carica papaya L.

, 2014
Souza, M., Gonsalves, D., Cai, W., Gonsalves, C., Zhu, H. Y., Jan, Fuh-Jyh, Fermin, G., Sarindu, N., Tennant, P.   +8 more
core  

Functional In Vitro Model of the Canine Corpus Luteum: Isolation, Culture and Characterization of Steroidogenically Active Luteal Cells. [PDF]

Biomedicines
Kalak P, Bugno P, Madej JP, Speruda M, Szumny A, Janeczek M, Niżański W, Gębarowski T, Dzięcioł M.   +8 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source