Results 231 to 240 of about 25,545 (268)

TECHNIQUE FOR COLLECTING MATURE HUMAN OOCYTES FOR IN VITRO FERTILIZATION

BJOG: An International Journal of Obstetrics & Gynaecology, 1981
SummaryMature oocytes may be obtained from 85 per cent of preovulatory follicles at laparoscopy. The technique of oocyte collection is described. The most important factors contributing to the success rate are surgical expertise and a satisfactory aspiration System.
C, Wood, J, Leeton, J M, Talbot, A O, Trounson   +3 more
openaire   +2 more sources

243 EFFECT OF OOCYTE RECOVERY TECHNIQUES ON IN VITRO MATURATION OF SWINE OOCYTES

Reproduction, Fertility and Development, 2008
The aim of this study was to compare 2 different techniques to obtain swine oocytes from abattoir ovaries. Ovaries were washed in saline at 35°C and submitted to slashing or aspiration, simultaneously. For the slashing group, ovaries were held with a hemostat inside a beaker containing 35 mL of HEPES-buffered Tyrode’s media (HbT) and follicles (2–6 mm)
F. R. O. de Barros, M. G. Marques, M. D. Goissis, M. A. Peres, M. P. Milazzotto, F. S. Maria, M. E. O. A. Assumpção, J. A. Visintin   +7 more
openaire   +1 more source

Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep

Small Ruminant Research, 2000
Three different methods, viz., puncture , aspiration and slicing were used for harvesting the oocytes of ovine ovaries. The aim was to determine an efficient method for oocyte harvesting and its effect on subsequent in vitro maturation and in vitro fertilization.
N.A Wani, G.M Wani, M.Z Khan, S Salahudin   +3 more
openaire   +1 more source

Influence of the collection technique of prepubertal goat oocytes on in vitro maturation and fertilization

Theriogenology, 1994
Prepubertal goat ovaries obtained from a slaughterhouse were used to study the influence of the oocyte collection technique (dissection, aspiration and slicing) on the number of oocytes recovered and their capacity for maturation and fertilization in vitro.
A, Martino, M J, Palomo, T, Mogas, M T, Paramio   +3 more
openaire   +2 more sources

Is in vitro maturation of oocytes retrieved ex vivo from ovarian tissue an effective fertility preservation technique in the presence of organic ovarian cysts?

European Journal of Obstetrics & Gynecology and Reproductive Biology, 2023
In vitro maturation (IVM) of oocytes retrieved ex vivo from ovarian tissue (OTO-IVM) could be an additional source of mature oocytes with the potential to optimise medical fertility preservation (FP) after oophorectomy. It is often undertaken at the same time as the ovarian tissue cryopreservation (OTC).
Bourg, Mona, Moreau, Jessika, Carles, Manon, Cadoret, Florence, Lesourd, Florence, Tournier, Anna, Léandri, Roger, Gatimel, Nicolas   +7 more
openaire   +3 more sources

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

Theriogenology, 1992
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were capacitated in isolated and ligated uterine horns of estrous gilts (Method A) for at least 3.5 hours and 1x10(6) sperm/ml were exposed to mature oocytes.
openaire   +2 more sources

Pregnancy and Calving Rates of Cloned Dromedary Camels Produced by Conventional and Handmade Cloning Techniques and In Vitro and In Vivo Matured Oocytes

Molecular Biotechnology, 2020
Despite practical implication of cloning in camelids, its broad application has been hampered by technical and biological problems. Method of somatic cell nuclear transfer (SCNT) and oocyte competence are the principal technical and biological factors, respectively, that determine the cloning efficiency. This study, therefore, investigated differential
F. Moulavi, B. Asadi-Moghadam, M. Omidi, M. Yarmohammadi, M. Ozegovic, A. Rastegar, S. M. Hosseini   +6 more
openaire   +2 more sources

Evaluation of Pre-maturity of Mouse Oocytes Ovulated from Prepubertal Females using an In Vitro Fertilization Technique

Journal of Mammalian Ova Research, 2006
ABSTRACT The cytogenetic normality and developmental competence of mouse oocytes derived from prepubertal females were investigated to determine ooplasmic maturity after in vitro fertilization (IVF) and to examine the ability of the resultant blastocysts to develop to term. To estimate the effect of body weight and age on ovulation, prepubertal female (
Akinori Mitsui, Midori Yoshizawa
openaire   +1 more source