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Tailor-made 3D in vitro maturation of early antral follicles uncovers cumulus-cell transcriptomic driver signature to predict oocyte competence [PDF]

open access: yesFrontiers in Endocrinology
Background Assisted reproductive technologies (ART) are constrained by the limited pool of medium antral follicles. Early antral follicles (EAfs) are an emerging additional source coming either from cryopreserved ovaries or from in vitro folliculogenesis
C. Rojo-Fleming   +12 more
semanticscholar   +2 more sources
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TECHNIQUE FOR COLLECTING MATURE HUMAN OOCYTES FOR IN VITRO FERTILIZATION

BJOG: An International Journal of Obstetrics & Gynaecology, 1981
SummaryMature oocytes may be obtained from 85 per cent of preovulatory follicles at laparoscopy. The technique of oocyte collection is described. The most important factors contributing to the success rate are surgical expertise and a satisfactory aspiration System.
C, Wood   +3 more
openaire   +2 more sources

243 EFFECT OF OOCYTE RECOVERY TECHNIQUES ON IN VITRO MATURATION OF SWINE OOCYTES

Reproduction, Fertility and Development, 2008
The aim of this study was to compare 2 different techniques to obtain swine oocytes from abattoir ovaries. Ovaries were washed in saline at 35°C and submitted to slashing or aspiration, simultaneously. For the slashing group, ovaries were held with a hemostat inside a beaker containing 35 mL of HEPES-buffered Tyrode’s media (HbT) and follicles (2–6 mm)
F. R. O. de Barros   +7 more
openaire   +1 more source

Influence of the collection technique of prepubertal goat oocytes on in vitro maturation and fertilization

Theriogenology, 1994
Prepubertal goat ovaries obtained from a slaughterhouse were used to study the influence of the oocyte collection technique (dissection, aspiration and slicing) on the number of oocytes recovered and their capacity for maturation and fertilization in vitro.
A, Martino   +3 more
openaire   +2 more sources

Exploring the impact of heat stress on oocyte maturation and embryo development in dairy cattle using a culture medium supplemented with vitamins E, C, and coenzyme Q10.

Journal of Thermal Biology, 2023
Heat stress is a significant factor affecting the fertility of dairy cattle due to the generation of free radicals. In assisted reproductive techniques, the inclusion of protective antioxidants becomes crucial to mitigate potential cellular damage.
Aref Maddahi   +4 more
semanticscholar   +1 more source

Research on vitro oocyte maturation technology and factors affecting oocyte maturation

International Conference on Biological Engineering and Medical Science
The development of oocytes is a vital process in living organisms, playing a crucial role in both human and animal health. This review aims to provide an in-depth exploration of oocyte morphogenesis, growth phases, maturation, and factors influencing ...
Borui Wang
semanticscholar   +1 more source

Is in vitro maturation of oocytes retrieved ex vivo from ovarian tissue an effective fertility preservation technique in the presence of organic ovarian cysts?

European Journal of Obstetrics & Gynecology and Reproductive Biology, 2023
In vitro maturation (IVM) of oocytes retrieved ex vivo from ovarian tissue (OTO-IVM) could be an additional source of mature oocytes with the potential to optimise medical fertility preservation (FP) after oophorectomy. It is often undertaken at the same time as the ovarian tissue cryopreservation (OTC).
Bourg, Mona   +7 more
openaire   +3 more sources

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

Theriogenology, 1992
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were capacitated in isolated and ligated uterine horns of estrous gilts (Method A) for at least 3.5 hours and 1x10(6) sperm/ml were exposed to mature oocytes.
openaire   +2 more sources

Pregnancy and Calving Rates of Cloned Dromedary Camels Produced by Conventional and Handmade Cloning Techniques and In Vitro and In Vivo Matured Oocytes

Molecular Biotechnology, 2020
Despite practical implication of cloning in camelids, its broad application has been hampered by technical and biological problems. Method of somatic cell nuclear transfer (SCNT) and oocyte competence are the principal technical and biological factors, respectively, that determine the cloning efficiency. This study, therefore, investigated differential
F. Moulavi   +6 more
openaire   +2 more sources

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