Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation. [PDF]
J Genet Eng Biotechnol, 2021 Munasinghe S +3 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Influence of calcium propionate on in vitro fermentation of sorghum-based diets
Revista de la Facultad de Ciencias Agrarias, 2017 The objective of the present study was to evaluate the effect of calcium propionate (CaPr) on in vitro ruminal fermentation using a factorial arrangement 2 x 2 evaluating CaPr (0 vs. 1%) and grain level (55 vs. 65%). There was a CaPr x Grain interaction
Luis Alberto Miranda +7 more
doaj
In vitro production and multiplex quantification of 2',3'-cyclic phosphate-containing 5'-tRNA half molecules. [PDF]
Methods, 2022 Kawamura T, Shigematsu M, Kirino Y.
europepmc +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2016 Linfeng Sun +3 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid.
ACS Synthetic Biology, 2016 Andrew R Hagen +6 more
semanticscholar +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
PROTEIN FRACTIONS AND IN VITRO FERMENTATION OF PROTEIN FEEDS FOR RUMINANTS
Tropical and Subtropical Agroecosystems, 2011 The objective of this study was to evaluate 20 protein feeds grouped in forages, vegetal by- products and animal by-products used for ruminant diets. Protein fractions (PF): A, non-protein nitrogen (NPN); B1, buffer-soluble protein; B2, buffer-insoluble,
Angel L. Guevara-Mesa +6 more
doaj
Correction: Babich et al. Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants. Molecules 2020, 25, 5805. [PDF]
Molecules, 2022 Babich O +5 more
europepmc +1 more source