Results 81 to 90 of about 222,372 (293)

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Monitoring the production of inclusion bodies during fermentation and enzyme-linked immunosorbent assay analysis of intact inclusion bodies using cryogel minicolumn plates

, 2006
A novel minicolumn chromatgraphic method to monitor the production of inclusion bodies during fermentation and anenzyme-linked immunosorbent assay (ELISA) system allowing direct analysis of the particles with surface-displayed antigens are described.
Dainiak, M.B., Ahlqvist, Josefin, Mattiasson, B., Mattiasson, Bo, Dainiak, Maria, Ahlqvist, Josefin,, Kumar, Ashok, Kumar, A., Hornsten, Gunnar E., Galaev, I.Yu., Galaev, Igor, Ahlqvist, J., Hornsten, E. Gunnar   +12 more
core   +1 more source

Successful production of recombinant buckwheat cysteine-rich aspartic protease in Escherichia coli [PDF]

Journal of the Serbian Chemical Society, 2009
Herein, the expression of recombinant cysteine-rich atypical buckwheat (Fagopyrum esculentum) aspartic protease (FeAPL1) in five Escherichia coli strains differing in their expression capabilities is presented.
MIRA D. MILISAVLJEVIĆ, DRAŽEN R. PAPIĆ, GORDANA S. TIMOTIJEVIĆ, VESNA R. MAKSIMOVIĆ   +3 more
doaj  

The human gut microbiome across the life course

FEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero, Basak Bahcivanci, Antonietta Hayhoe, Animesh Acharjee, Ezgi Özkurt   +4 more
wiley   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Bacterial Inclusion Bodies: Discovering Their Better Half.

, 2017
Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant bacteria showing analogies with secretory granules of the mammalian endocrine system.
Corchero, José Luis, Garcia-Fruitós, Elena, Vázquez, Esther, Antonio Villaverde, Seras-Franzoso, Joaquin, Villaverde, Antonio, José Luis Corchero, Elena Garcia-Fruitós, Esther Vázquez, Joaquin Seras-Franzoso, Rinas, Ursula, Ursula Rinas   +11 more
core   +1 more source

Chemical Assistance in Refolding of Bacterial Inclusion Bodies

Biochemistry Research International, 2011
Escherichia coli is one of the most widely used hosts for the production of recombinant proteins but insoluble expression of heterologous proteins is a major bottleneck in production of recombinant proteins in E. coli.
Mona Alibolandi, Hasan Mirzahoseini
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Inclusion Bodies

, 1941
In order that we may both have in mind the same thing during this paper it is probably well to set forth a brief description of inclusion bodies. In a broad biological sense the term applies to any formed mass of material, such as secretion granules and ...
Lucas, Alfred
core   +1 more source