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Partitioning Indicator Displacement Assay [PDF]

open access: yesACS Omega
Indicator displacement assays work by detecting the replacement of an indicator from a binding site by an analyte. The binding sites are typically located on individual receptor molecules capable of carrying out molecular recognition. Here, we describe a new transduction mechanism based on analyte partitioning in a two-phase system, which we refer to ...
Vincent Tocci   +5 more
doaj   +3 more sources

Fluorescence Detection of Deoxyadenosine in Cordyceps spp. by Indicator Displacement Assay [PDF]

open access: yesMolecules, 2020
A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established.
Arinta Agnie Dewantari   +6 more
doaj   +5 more sources

Development of a New Colorimetric Chemosensor for Selective Determination of Urinary and Vegetable Oxalate Concentration Through an Indicator Displacement Assay (IDA) in Aqueous Media [PDF]

open access: yesFood Technology and Biotechnology, 2018
The paper proposes a method that exhibits operational simplicity for the indirect spectrophotometric determination of oxalate ion. We developed Reactive Blue 4 (RB4) as a sensor by complexation with copper ion as a simple, inexpensive yet selective ...
Hossein Tavallali   +2 more
doaj   +2 more sources

A Saccharide Chemosensor Array Developed Based on an Indicator Displacement Assay Using a Combination of Commercially Available Reagents [PDF]

open access: yesFrontiers in Chemistry, 2019
Herein, a very simple colorimetric chemosensor array is reported for saccharides (D-glucose, D-fructose, D-xylose, D-galactose, D-mannose, L-rhamnose, and N-acetyl-D-gluosamine).
Yui Sasaki   +2 more
doaj   +2 more sources

A Highly Selective and Strong Anti-Interference Host-Guest Complex as Fluorescent Probe for Detection of Amantadine by Indicator Displacement Assay [PDF]

open access: yesMolecules, 2018
Amantadine (AMA) and its derivatives are illicit veterinary drugs that are hard to detect at very low concentrations. Developing a fast, simple and highly sensitive method for the detection of AMA is highly in demand.
Linzhao Zhu   +5 more
doaj   +2 more sources

An indicator displacement assay with independent dual wavelength emission. [PDF]

open access: yesTetrahedron Lett, 2010
A multifunctional metallo-receptor was designed with both metal and boronic acid binding groups. A sensor ensemble was prepared using the metallo-receptor and the fluorescent dye ARS. The dye produced two distinct fluorescent bands from interaction with the boronic acid and the metal respectively.
Zhang S, Glass TE.
europepmc   +4 more sources

Carboxylato-Pillar[6]arene-Based Fluorescent Indicator Displacement Assays for Caffeine Sensing [PDF]

open access: yesFrontiers in Chemistry, 2021
In the present work, we have developed a new indicator displacement system based on pillararene for anionic water-soluble carboxylato pillar [6] arene (WP6) and aromatic fluorescent dye safranine T (ST). A large fluorescence enhancement and colour change
Qunpeng Duan, Yibo Xing, Kainan Guo
doaj   +3 more sources

Recent advances in macrocyclic arenes-based fluorescent indicator displacement assays

open access: yesFrontiers in Chemistry, 2022
Macrocyclic arenes-based fluorescent indicator displacement assays (F-IDAs) offer a unique and innovative approach to chemosensing, taking molecular recognition in host-guest chemistry to a higher level.
Qunpeng Duan, Fei Wang, Kui Lu, Kui Lu
doaj   +3 more sources

Cucurbit[n]uril-based fluorescent indicator-displacement assays for sensing organic compounds

open access: yesFrontiers in Chemistry, 2023
The widespread conversion of synthetic receptors into luminescent sensors has been achieved via the use of fluorescent-indicator displacement assays (F-IDAs). Due to their rigid structures and efficient binding affinities, cucurbit[n]urils, combined with
Qunpeng Duan   +11 more
doaj   +3 more sources

Measurement of precipitated calcium citrate in 24-hour urine samples is clinically useful for ruling out calcium oxalate urolithiasis [PDF]

open access: yesScience Progress
Objective: Combining calcium with citrate clinically prevents the formation of calcium oxalate (CaOx) stones. In this study, we developed a new method for measuring calcium citrate (CalCit) precipitated out of urine samples.
Chaiyadol Tantasith   +4 more
doaj   +2 more sources

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