Results 51 to 60 of about 46,142 (281)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A colorimetric competitive displacement assay for the evaluation of catalytic peptides [PDF]

, 2017
An assay based on competitive dye displacement was adapted to detect the formation of aldol product in crude reaction mixtures, and was used to evaluate minimal peptide aldol catalysts.
Aguiluz, Erika M., Gest, Anneliese, Liu, Xinyu, Mays, Malik T., Neidhart, Eliza K., Witus, Leah S.   +5 more
core   +2 more sources

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of Mycoplasma pneumoniae

Frontiers in Cellular and Infection Microbiology, 2019
Mycoplasma pneumoniae (M. pneumoniae) is responsible for pneumonia, and is a causative agent of other respiratory tract infections (e.g., bronchiolitis and tracheobronchitis). Herein, we established and applied a multiple cross displacement amplification
Yacui Wang, Yi Wang, Shuting Quan, Weiwei Jiao, Jieqiong Li, Lin Sun, Yonghong Wang, Xue Qi, Xingyun Wang, Adong Shen   +9 more
doaj   +1 more source

Binding Modes of a Phenylpyridinium Styryl Fluorescent Dye with Cucurbiturils

Molecules, 2020
In order to explore how cucurbituril hosts accommodate an N-phenyl-pyridinium derivative guest, the complexation of the solvatochromic dye, 4-(4-(dimethylamino)styryl)-1-phenylpyridinium iodide (PhSt) with α,α′,δ,δ′-tetramethyl-cucurbit[6]uril (Me4CB6 ...
Adrien Paudics, Dóra Hessz, Márton Bojtár, Benjámin Gyarmati, András Szilágyi, Mihály Kállay, István Bitter, Miklós Kubinyi   +7 more
doaj   +1 more source

Synthesis of 2-Methyl-3-indolylacetic Derivatives as Anti-Inflammatory Agents That Inhibit Preferentially Cyclooxygenase 1 without Gastric Damage [PDF]

, 2006
Novel substituted 2-methyl-3-indolylacetic derivatives were synthesized and evaluated for their activity in vitro and in vivo on COX-1 and COX-2. Active compounds were screened to determine their gastrointestinal tolerability in vivo in the rat.
C. Renner, CALIENDO, GIUSEPPE, CIRINO, GIUSEPPE, FIORINO, FERDINANDO, PERISSUTTI, ELISA, ROSSI, ANTONIETTA, ROVIEZZO, FIORENTINA, SANTAGADA, VINCENZO, SAUTEBIN, LIDIA, SEVERINO, BEATRICE   +9 more
core   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Fluorescent Probe for δ‐Cyclodextrin Enables Guest Encapsulation Studies via an Indicator Displacement Assay

ChemistryEurope
Molecular containers are important tools to enhance the solubility and stability of bioactive compounds in water, and new macrocyclic scaffolds will expand the range of guests that can be encapsulated.
Mads G. Rasmussen, Giorgia Masciotta, Kasper H. Hansen, Andreas Erichsen, Gustav Schjær Hummelshøj, Panagiota Kalomoiri, Sebastian Meier, Sophie R. Beeren   +7 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

A Multimode Detection Platform for Biothiols Using BODIPY Dye-Conjugated Gold Nanoparticles

Colorants
This study explored the synthesis and application of BODIPY-functionalized gold nanoparticles (AuNPs) for the sensitive detection of biothiols via an indicator displacement assay coupled with surface-enhanced Raman scattering (SERS) techniques, alongside
Panangattukara Prabhakaran Praveen Kumar
doaj   +1 more source