Results 31 to 40 of about 64,392 (219)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Dissecting and steering cell dynamics using spatially-informed RNA velocity with veloAgent

Molecular Systems Biology
RNA velocity enables inference of cell state transitions from single-cell transcriptomics by modeling transcriptional dynamics from spliced and unspliced mRNA.
Vishvak Raghavan, Brent Yoon, Gregory J Fonseca, Yue Li, Jun Ding   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

KSRV: a Kernel PCA-Based framework for inferring spatial RNA velocity at single-cell resolution

Frontiers in Genetics
Understanding the temporal dynamics of gene expression within spatial contexts is essential for deciphering cellular differentiation. RNA velocity, which estimates the future state of gene expression by distinguishing spliced from unspliced mRNA, offers ...
Yan He, Yan He, Jian Jiang, Jian Jiang, Huahai Qiu, Huahai Qiu, Ya-Zhou Shi, Ya-Zhou Shi, Ben-Gong Zhang, Ben-Gong Zhang   +9 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Reconstructing angular light field by learning spatial features from quadrilateral epipolar geometry

Scientific Reports
Recent research on dense multi-view image reconstruction has attracted considerable attention, due to its enhancement of applications such as 3D reconstruction, de-occlusion, depth sensing, saliency detection, and prominent object identification.
Ebrahem Elkady, Ahmed Salem, Hyun-Soo Kang, Jae-Won Suh   +3 more
doaj   +1 more source

Local rabies transmission and regional spatial coupling in European foxes.

PLoS ONE, 2020
Infectious diseases are often transmitted through local interactions. Yet, both surveillance and control measures are implemented within administrative units.
Laurie Baker, Jason Matthiopoulos, Thomas Müller, Conrad Freuling, Katie Hampson   +4 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source