Results 191 to 200 of about 114,208,131 (218)
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Applied Microbiology and Biotechnology, 2011
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong +11 more
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Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong +11 more
openaire +3 more sources
The Southeast Asian journal of tropical medicine and public health, 2010
Neuraminidase (NA) is an envelope surface glycoprotein of influenza A viruses. It cleaves alpha-(2,3) or alpha-(2,6) glycosidic linkage between a terminal sialic acid residue of the host cell receptor and hemagglutinin of the viral envelope, thus releasing viral progeny from the infected cell.
Anchalee, Rawangkhan +7 more
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Neuraminidase (NA) is an envelope surface glycoprotein of influenza A viruses. It cleaves alpha-(2,3) or alpha-(2,6) glycosidic linkage between a terminal sialic acid residue of the host cell receptor and hemagglutinin of the viral envelope, thus releasing viral progeny from the infected cell.
Anchalee, Rawangkhan +7 more
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Development of a recombinant fowlpox virus vector-based vaccine of H5N1 subtype avian influenza.
Developments in biologicals, 2006The genetic stability of the recombinant fowlpox virus (named rFPV-HA-NA) was confirmed by serial passage on chicken embryo fibroblast (CEF) cells. The immune efficacy, safety, the minimum immunising dose, the time of immunity induced and the immune duration of the vector-based vaccine was evaluated in specific-pathogen-free (SPF) chickens.
C, Qiao +6 more
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2009
One highly pathogenic strain of avian influenza virus (AIV) was isolated from goose in China recently, designated as F-3. In order to study the viral entry mechanisms, the hemagglutinin (HA) gene of H5N1 subtype AIV isolate was amplified by RT-PCR, and then cloned into pGEM-T vector and sequenced.
Hua-Lei, Liu +5 more
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One highly pathogenic strain of avian influenza virus (AIV) was isolated from goose in China recently, designated as F-3. In order to study the viral entry mechanisms, the hemagglutinin (HA) gene of H5N1 subtype AIV isolate was amplified by RT-PCR, and then cloned into pGEM-T vector and sequenced.
Hua-Lei, Liu +5 more
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Advances in Detection Techniques for the H5N1 Avian Influenza Virus
International Journal of Molecular Sciences, 2023Xian-Shu Fu, Sun Kai, Biao Zhang
exaly
The Egyptian journal of immunology, 2012
Influenza A virus continue to cause widespread morbidity and mortality. The unprecedented spread of highly pathogenic avian influenza virus subtype H5N1 in Egypt is threatening poultry and public health systems. Effective diagnosis and control management are needed to control the disease. To this end, polyclonal antibodies (PAbs) were developed against
M H, Kdodier, S A, Shoman
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Influenza A virus continue to cause widespread morbidity and mortality. The unprecedented spread of highly pathogenic avian influenza virus subtype H5N1 in Egypt is threatening poultry and public health systems. Effective diagnosis and control management are needed to control the disease. To this end, polyclonal antibodies (PAbs) were developed against
M H, Kdodier, S A, Shoman
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Wei sheng wu xue bao = Acta microbiologica Sinica, 2006
The HA connecting peptide at cleavage site, PQRERRKKR / GL, of an H5N1 subtype avian influenza virus (AIV) was replaced with PQRESR / GL, and then the modified HA gene was cloned into the transcription/expression vector, pHW2000, constructing a plasmid named pHW524-HA. The NA (N1) gene from the H5N1 virus and the NA (N2) gene from an H9N2 AIV were also
Jian-hong, Lu +4 more
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The HA connecting peptide at cleavage site, PQRERRKKR / GL, of an H5N1 subtype avian influenza virus (AIV) was replaced with PQRESR / GL, and then the modified HA gene was cloned into the transcription/expression vector, pHW2000, constructing a plasmid named pHW524-HA. The NA (N1) gene from the H5N1 virus and the NA (N2) gene from an H9N2 AIV were also
Jian-hong, Lu +4 more
openaire +1 more source
Temporal Dynamics of Influenza A(H5N1) Subtype before and after the Emergence of H5N8
Viruses, 2021Mahmoud Naguib +2 more
exaly
Phylogenetic and computational proteome analysis of Influenza A virus subtype H5N1
The Internet Journal of Genomics and Proteomics, 2008openaire +1 more source
The role of house flies (Musca Domestica) in transmission of avian influenza virus Subtype H5N1
The objective of this study was to determine the potential role of the house flies in harboring and transmission avian influenza virus H5N1 (AIVs). To achieve this goal, this study was divided into three parts. The purpose of the first part was to determine the potential of the house flies as a vector of AIVs.openaire +1 more source