Results 71 to 80 of about 13,032 (260)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Channel Quality-Based Optimal Status Update for Information Freshness in Internet of Things. [PDF]

Entropy (Basel), 2021
Peng F, Chen X, Wang X.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Proportional Fair Information Freshness Under Jamming

, 2019
The success of a UAV mission depends on communication between a GCS (Ground Control Station) and a group of UAVs. It is essential that the freshness of the commands received by UAVs is maintained as mission parameters often change during an operation. Ensuring the freshness of the commands received by UAVs becomes more challenging when operating in an ...
Andrey Garnaev, Jing Zhong, Wuyang Zhang, Roy D. Yates, Wade Trappe   +4 more
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Multi-Stage Structured Estimators for Information Freshness

CoRR
Most of the contemporary literature on information freshness solely focuses on the analysis of freshness for martingale estimators, which simply use the most recently received update as the current estimate. While martingale estimators are easier to analyze, they are far from optimal, especially in pull-based update systems, where maximum aposteriori ...
Sahan Liyanaarachchi, Sennur Ulukus, Nail Akar   +2 more
openaire   +2 more sources

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Structured Estimators: A New Perspective on Information Freshness

2025 IEEE Information Theory Workshop (ITW)
In recent literature, when modeling for information freshness in remote estimation settings, estimators have been mainly restricted to the class of martingale estimators, meaning the remote estimate at any time is equal to the most recently received update. This is mainly due to its simplicity and ease of analysis.
Sahan Liyanaarachchi, Sennur Ulukus, Nail Akar   +2 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Can Consumers Judge the Freshness of Fish from Visual Cues? A Case Study of Japanese Consumers

Foods
In contemporary markets, fish are frequently wrapped in cling film, necessitating consumers’ reliance on visual cues to assess freshness. This study explores whether common Japanese consumers can accurately discern fish freshness based solely on visual ...
Penglong Li, Yutaro Sakai, Hisashi Kurokura, Nobuyuki Yagi   +3 more
doaj   +1 more source