Results 91 to 100 of about 2,120,304 (301)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Energy-Aware Joint User Association and Resource Allocation for Coded Cache-Enabled HetNets

open access: yesIEEE Access, 2019
Cache-enabled heterogeneous networks (HetNets) have recently emerged as an attractive solution to meet the exponentially increasing demand on mobile data traffic.
Fangfang Yin   +3 more
doaj   +1 more source

Cooperative localization by dual foot-mounted inertial sensors and inter-agent ranging

open access: yes, 2013
The implementation challenges of cooperative localization by dual foot-mounted inertial sensors and inter-agent ranging are discussed and work on the subject is reviewed. System architecture and sensor fusion are identified as key challenges. A partially
Händel, Peter   +3 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Replicated Secure Shared Objects for Groupware Applications [PDF]

open access: yes, 1995
This report describes the design and implementation of a secure shared replicated objects system, intended for use by groupware application programmers. The system enables such programmers to incorporate conceptually shared objects into their programs in
Dollimore, Jean, Rowley, Andrew
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Characterizing and Predicting the Popularity of Online Videos

open access: yesIEEE Access, 2016
A huge amount of video content has been generated on the Internet, and user attention among those videos is allocated in an asymmetric way, with the vast majority barely noticed while a few of videos become very popular.
Chenyu Li, Jun Liu, Shuxin Ouyang
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Game Theory Based Energy-Aware Uplink Resource Allocation in OFDMA Femtocell Networks

open access: yesInternational Journal of Distributed Sensor Networks, 2014
Femtocell is a promising technique not only in operator networks but also in having potential applications for industrial wireless sensor networks. In this paper, we investigate energy efficient uplink power control and subchannel allocation in two-tier ...
Jun Zhao   +5 more
doaj   +1 more source

Intercompartmental communication in senescence

open access: yesFEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz   +3 more
wiley   +1 more source

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