Results 211 to 220 of about 31,115,988 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Genome-Wide Identification of the Glycosyltransferase 4 Family and Characterization of <i>GmSPS8</i> Associated with Seed Weight in Soybean. [PDF]

Curr Issues Mol Biol
Cao H, Liu L, Zhang K, Ma S, Wang M, Sun Y, Zhuang Y, Chen B, Zhang J, Zhang D, Li X.   +10 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

A group-based hands-on seminar for teaching failure mode and effects analysis in CyberKnife stereotactic radiotherapy. [PDF]

J Appl Clin Med Phys
Okamoto H, Nishioka S, Kamomae T, Okawa K, Kikuchi S, Suzuki J.   +5 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Retraction: Economic impact of a vision-based patient monitoring system across five NHS mental health trusts. [PDF]

PLOS Digit Health
PLOS Digital Health Editors.
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Comparative Analysis of Algorithms for Extracting Frames from Unexplored Regions in Molecular Dynamics Simulations. [PDF]

ACS Omega
Nagare A, Wagh S, Kotipalli A, Koulgi S, Jani V, Achalere A, Kale M, Sonavane U.   +7 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Signal-Driven Model Order Selection for MUSIC-Based HRV Spectral Characterization. [PDF]

Bioengineering (Basel)
Garza-Barrón PL, Barrientos-García A, Lastre-Domínguez CM, Rivera-Romero CA, Villanueva-Maldonado J, Muñoz-Minjares JU.   +5 more
europepmc   +1 more source