Results 311 to 320 of about 212,226 (348)

Proteomics of mitochondrial inner and outer membranes

PROTEOMICS, 2008
Abstract For the proteomic study of mitochondrial membranes, documented high quality mitochondrial preparations are a necessity to ensure proper localization. Despite the state‐of‐the‐art technologies currently in use, there is no single technique that can be used for all studies of mitochondrial membrane proteins.
Anne M, Distler, Janos, Kerner, Charles L, Hoppel   +2 more
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Modulation of inner mitochondrial membrane channel activity

Journal of Bioenergetics and Biomembranes, 1992
Three classes of inner mitochondrial membrane (IMM) channel activities have been defined by direct measurement of conductance levels in membranes with patch clamp techniques in 150 mM KCl. The "107 pS activity" is slightly anion selective and voltage dependent (open with matrix positive potentials).
K W, Kinnally, Y N, Antonenko, D B, Zorov   +2 more
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Permeability of inner mitochondrial membrane and oxidative stress

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1988
The mechanism of increase in the inner membrane permeability induced by Ca2+ plus Pi, diamide and hydroperoxides has been analyzed. (1) The permeability increase is antagonized by oligomycin and favoured by atractyloside. The promoting effect of atractyloside is strongly reduced if the mitochondria are simultaneously treated with oligomycin.
CARBONERA, DONATELLA, AZZONE GF
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Calcium modulation of mitochondrial inner membrane channel activity

Biochemical and Biophysical Research Communications, 1991
Protocols were defined that enable patch-clamp studies of the approximately 107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10(-7) M or lower during mitochondrial isolation, no activity was
K W, Kinnally, D, Zorov, Y, Antonenko, S, Perini   +3 more
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Voltage activation of heart inner mitochondrial membrane channels

Journal of Bioenergetics and Biomembranes, 1992
The patch clamp records obtained from mitoplast membranes prepared in the presence of a calcium chelator generally lack channel activity. However, multiconductance channel (MCC) activity can be induced by membrane potentials above +/- 60 mV [Kinnally et al., Biochem. Biophys. Res. Commun. 176, 1183-1188 (1991)].
D B, Zorov, K W, Kinnally, H, Tedeschi
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The ribbon structure of the mitochondrial inner membrane

Journal of Bioenergetics and Biomembranes, 1977
Electron microscopic evidence is presented for the extensive association of protein subunits into ribbons within the mitochondrial inner membrane. The mitochondrial cristae can be rearranged to a narrow tubular form which exhibits ribbon structure and is fully functional; the morphology of particles derived from sub-mitochondrial electron transport ...
R A, Haworth, H, Komai, D E, Green, W J, Vail   +3 more
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Metallothionein-Induced Increase in Mitochondrial Inner Membrane Permeability

Journal of Surgical Research, 1998
To elucidate the effect of metallothionein on the permeability of the mitochondrial inner membrane.Metallothionein (MT) is a 6-7-kDa protein which is rapidly produced by stressed cells. MT is induced by cytokines and other factors thought to mediate the stress response. The organelle with the highest concentration of MT is the mitochondrion.
C, Simpkins, T, Lloyd, S, Li, S, Balderman   +3 more
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Mitochondrial inner membrane changes determined by cross partition

Biochemical and Biophysical Research Communications, 1975
Intact beef heart mitochondria and submitochondrial particles have different isopartition points. For intact mitochondria a cross-point is obtained at pH 5.6 and for submitochondrial particles at pH 6.6. ATP but not necessarily ITP lowers the isopartition point in submitochondrial particles, indicating conformational and/or charge changes in the ...
P, Lundberg, I, Ericson
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Translocation of mitochondrial inner-membrane proteins: conformation matters

Trends in Biochemical Sciences, 2006
Most of the mitochondrial inner-membrane proteins are generated without a presequence and their targeting depends on inadequately defined internal segments. Despite the numerous components of the import machinery identified by proteomics, the properties of hydrophobic import substrates remain poorly understood. Recent studies support several principles
de Marcos-Lousa, Carine, Sideris, Dionisia P., Tokatlidis, Kostas   +2 more
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The proton leak across the mitochondrial inner membrane

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
The proton conductance of the mitochondrial inner membrane increases at high protonmotive force in isolated mitochondria and in mitochondria in situ in rat hepatocytes. Quantitative analysis of its importance shows that about 20-30% of the oxygen consumption by resting hepatocytes is used to drive a heat-producing cycle of proton pumping by the ...
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