Results 101 to 110 of about 468,532 (266)

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Long-lasting and stable anti-fog coating combined with active and passive strategy

Nature Communications
There is an urgent need to design long-term effective anti-fog coatings to ensure the stabilization of uniform water films on hydrophilic surfaces, considering the impacts of interfacial strength, cyclic drying, and contaminants. Here, we propose an anti-
Cijian Zhang, Beitao Liu, Shengyuan Yu, Jiahui Li, Qian Liu, Shouhai Zhang, Xigao Jian, Zhihuan Weng   +7 more
doaj   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Janus structure breaks the solar incidence angular constraint insu photothermal anti-icing

Nature Communications
Angle-sensitive sunlight absorption in state-of-the-art photothermal anti-icing surfaces becomes an Achilles’ heel: geometric shadowing and Fresnel reflection critically degrade broadband harvesting under oblique (incident light >45°) or low solar ...
Cijian Zhang, Beitao Liu, Yang Chen, Leiming Sun, Meiyu Yan, Qian Liu, Ce Song, Shouhai Zhang, Jinlong Song, Cheng Liu, Jinyan Wang, Xigao Jian, Zhihuan Weng   +12 more
doaj   +1 more source

Simplified calculation method for the critical load bearing of market-based profile emergency steel pier

Heliyon
A novel emergency steel pier, assembled from market-available profiles, has been developed to address the challenges associated with the ''regular stockpiling and rapid emergency deployment'' of bridge pier repair equipment.
Zi-yu Liu, Shi-tong Chen, Mo-mo Zhi, Kai Zhang, Hong-tao Zhang   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The use of a novel toe–thumb pressure index for assessing arterial status in the lower limb. A reliability and validity study

Journal of Foot and Ankle Research
Aims This study explored the reliability, validity and perceived comfort of a novel thumb pressure measure and calculation of a toe–thumb index to identify their suitability as an adjunct or alternatives to ankle–brachial and toe–brachial indices ...
Juliana Mazzeo, Helen A. Banwell, Peta E. Tehan, Grace Anderson, Kristin Graham   +4 more
doaj   +1 more source