Results 11 to 20 of about 14,200 (182)

Massively parallel single-nucleotide mutagenesis using reversibly terminated inosine [PDF]

Nature Methods, 2016
Large-scale mutagenesis of target DNA sequences allows researchers to comprehensively assess the effects of single-nucleotide changes. Here we demonstrate the construction of a systematic allelic series (SAS) using massively parallel single-nucleotide mutagenesis with reversibly terminated deoxyinosine triphosphates (rtITP).
Gabe Haller, David Alvarado, Kevin McCall, Robi D Mitra, Matthew B Dobbs, Christina A Gurnett   +5 more
openaire   +2 more sources

Effective and repeatable chromatographic separation of 5 nucleotides in infant formula milk powder by ion-pair high-performance liquid chromatography–ultraviolet

Journal of Dairy Science, 2022
: A robust method using HPLC-UV was developed to improve the accuracy and repeatability of a quantitative method to detect 5 nucleotides (cytidine-monophosphate, uridine monophosphate, adenosine monophosphate, guanine monophosphate, and inosine ...
Yuanjia Chen, Lin Luo, Peiyan Feng, Baojun Xu, Xiaoqun Wei   +4 more
doaj   +1 more source

Identification of Umami Taste in Sous-Vide Beef by Chemical Analyses, Equivalent Umami Concentration, and Electronic Tongue System

Foods, 2020
Behaviour of umami compounds that are associated with non-volatile compounds on slow cooking regimes remains less explored. This study aims to assess the ability of the electronic tongue system on the umami taste from sous-vide beef semitendinosus.
Young-Hwa Hwang, Ishamri Ismail, Seon-Tea Joo   +2 more
doaj   +1 more source

Effects of dietary supplementation of nucleotides from late gestation to lactation on the performance and oxidative stress status of sows and their offspring

Animal Nutrition, 2021
Increased metabolic burdens in breeding sows, which are induced by elevated systemic oxidative stress, could increase the need for nucleotides to repair lymphocyte DNA damage; however, de novo synthesis of nucleotides may be insufficient to cover this ...
Chengquan Tan, Yongcheng Ji, Xichen Zhao, Zhongquan Xin, Jiaying Li, Shuangbo Huang, Zhiying Cui, Lijun Wen, Caihua Liu, Sung Woo Kim, Jinping Deng, Yulong Yin   +11 more
doaj   +1 more source

CAQ Corner: Basic concepts of transplant immunology

, 2022
Liver Transplantation, EarlyView.
Amanda Cheung, Josh Levitsky
wiley   +1 more source

Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

Nature Communications, 2020
Trypanosoma brucei guanosine 5′-monophosphate reductase (TbGMPR) catalyses the conversion of GMP to inosine 5′-monophosphate and contains a cystathionine-β-synthase (CBS) domain. Here the authors combine X-ray crystallography and kinetic measurements and
Akira Imamura, Tetsuya Okada, Hikaru Mase, Takuya Otani, Tomoka Kobayashi, Manatsu Tamura, Bruno Kilunga Kubata, Katsuaki Inoue, Robert P. Rambo, Susumu Uchiyama, Kentaro Ishii, Shigenori Nishimura, Takashi Inui   +12 more
doaj   +1 more source

Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods.

PLoS ONE, 2022
Nutritional value of black soldier fly (Hermetia illucens) larvae (BSFL) processed by three different methods of treatment was compared. The resulting products were the spray-dried BSFL (SPR), oven-dried BSFL 1 (OVN1) and oven-dried BSFL 2 (OVN2 ...
Nor Fatin Najihah Mohamad Zulkifli, Annita Yong Seok-Kian, Lim Leong Seng, Saleem Mustafa, Yang-Su Kim, Rossita Shapawi   +5 more
doaj   +1 more source

Untargeted Metabolomics Analysis using LC-MSQTOF for Metabolite Profile Comparison between Patients with Myofascial Pain of Upper Trapezius Muscle versus Controls

Siriraj Medical Journal, 2022
Objective: This study aims to identify different biomarkers of Myofascial pain syndrome (MPS) using untargeted metabolomics screening. Materials and Methods: In a case-control study, serum samples from MPS patients (n = 19) and healthy controls (n = 10)
Manmas Vannabhum, Kamontip Harnphadungkit, Pravit Akarasereenont, Sompol Tapechum   +3 more
doaj   +1 more source

Nucleotide degradation and functional impairment during cardioplegia: amelioration by inosine. [PDF]

Circulation, 1983
The degradation of adenine nucleotide levels and impairment of functional recovery associated with exposure to hypothermic (20 degrees C) cardioplegia was studied in 84 isolated working rat hearts. After a 1-hour control period, hearts were exposed to 1 hour of cardioplegia that consisted of increasingly longer periods of cardioplegic solution (CPS ...
D F, DeWitt, K E, Jochim, D M, Behrendt
openaire   +2 more sources

Control of ATP homeostasis during the respiro‐fermentative transition in yeast

Molecular Systems Biology, 2010
Respiring Saccharomyces cerevisiae cells respond to a sudden increase in glucose concentration by a pronounced drop of their adenine nucleotide content ([ATP]+[ADP]+[AMP]=[AXP]).
Thomas Walther, Maite Novo, Katrin Rössger, Fabien Létisse, Marie‐Odile Loret, Jean‐Charles Portais, Jean‐Marie François   +6 more
doaj   +1 more source