Results 61 to 70 of about 617,921 (305)

Shared midgut binding sites for Cry1A.105, Cry1Aa, Cry1Ab, Cry1Ac and Cry1Fa proteins from Bacillus thuringiensis in two important corn pests, Ostrinia nubilalis and Spodoptera frugiperda. [PDF]

PLoS ONE, 2013
First generation of insect-protected transgenic corn (Bt-corn) was based on the expression of Cry1Ab or Cry1Fa proteins. Currently, the trend is the combination of two or more genes expressing proteins that bind to different targets.
Carmen Sara Hernández-Rodríguez, Patricia Hernández-Martínez, Jeroen Van Rie, Baltasar Escriche, Juan Ferré   +4 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Bacillus thuringiensis delta-endotoxin Cry1 hybrid proteins with increased activity against the Colorado potato beetle

, 2001
Cry1 delta-endotoxins of Bacillus thuringiensis are generally active against lepidopteran insects, but Cry1Ba and Cry1Ia have additional, though low, levels of activity against coleopterans such as the Colorado potato beetle.
Naimov, S., de Maagd, R.A., Dukiandjiev, S., Maagd, R.A., de, Weemen-Hendriks, M.   +4 more
core   +1 more source

Production of recombinant proteins in insect cells

, 2022
S.255-271Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive posttranslational modification. This review
Vilcinskas, A., Kollewe, C.
core   +1 more source

Allergenic potential of insect proteins: cross-reactivity, detection, degradation, and implications for food safety and labelling

Food Science of Animal Products
The growing global demand for protein, driven by population expansion, has highlighted the need for sustainable and nutritionally rich alternatives. Insect protein presents a promising solution, offering a high-quality, environmentally sustainable source
Dongdong Ni
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Biochemical basis of insect resistance in winged bean (Psophocarpus tetragonolbus): characterisation of insecticidal proteins and their encoding genes [PDF]

, 1991
Many pulses and beans grown for human comsumption are susceptible to insect attack. Winged bean, a high protein crop of the tropics, yield seeds which appear to be immune to infestation by the storage bruchid Callosobruchus maculatus.
Howe, David S.
core  

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Digestive enzymes of vine weevil (Otiorhynchus sulcatus) as potential targets for insect control strategies. [PDF]

, 2002
Over the previous quarter century the vine weevil (Otiorhynchus sulcatus) has become a pest of horticultural and agricultural plants. The vine weevil is a polyphagous coleopteran insect and is able to attack over one hundred different plant species.
Edwards, M.G., Edwards, Martin Gethin
core  

Evolutionary plasticity of insect immunity

, 2022
S.123-129Many insect genomes have been sequenced and the innate immune responses of several species have been studied by transcriptomics, inviting the comparative analysis of immunity-related genes. Such studies have demonstrated significant evolutionary
Vilcinskas, A.
core   +1 more source