Results 1 to 10 of about 595 (210)
Long-Distance Effects of Insertional Mutagenesis [PDF]
PLoS ONE, 2011 Most common systems of genetic engineering of mammalian cells are associated with insertional mutagenesis of the modified cells. Insertional mutagenesis is also a popular approach to generate random alterations for gene discovery projects.
Eugene S Kandel
exaly +5 more sources
Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics
Plants, 2019 Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated ...
Hasthi Ram +2 more
exaly +3 more sources
Insertional Mutagenesis of Chlamydomonas reinhardtii
Bio-Protocol, 2015 The unicellular microalga Chlamydomonas reinhardtii (C. reinhardtii) has been used as a reference model for numerous fields of research. Principle research areas are eukaryotic flagellar structure and function, basal bodies (centrioles), cell-cell ...
Chia-Hong Tsai, Christoph Benning
doaj +2 more sources
Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens [PDF]
PLoS Computational Biology, 2006 Retroviral insertional mutagenesis screens, which identify genes involved in tumor development in mice, have yielded a substantial number of retroviral integration sites, and this number is expected to grow substantially due to the introduction of high ...
Jeroen De Ridder +2 more
exaly +2 more sources
Insertional mutagenesis in zebrafish [PDF]
Developmental Dynamics, 2003 AbstractInsertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus‐mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes ...
Adam Amsterdam, Nancy Hopkins
openaire +3 more sources
Insertional mutagenesis strategies in zebrafish [PDF]
Genome Biology, 2007 We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and ...
Sivasubbu, Sridhar +3 more
openaire +3 more sources
Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. [PDF]
PLoS ONE, 2013 To investigate mechanisms and phenotypic effects of insertional mutagenesis by gammaretroviruses, we have developed mouse lines containing a single Akv 1-99 long terminal repeat (LTR) and a floxed PGK/Tn5 neomycin cassette at the Nras proto-oncogene at ...
Borja Ballarín-González +5 more
doaj +1 more source
Transposon Insertional Mutagenesis in Rice, [PDF]
Plant Physiology, 2001 Transposon mutagenesis systems are being developed in rice to address gene functions using forward and reverse genetics strategies. Although the well-characterized maize ( Zea mays ) transposons seem an obvious choice to develop efficient transposon-tagging systems for gene knockouts and ...
Greco, R. +9 more
openaire +5 more sources
In vivo insertion pool sequencing identifies virulence factors in a complex fungal-host interaction. [PDF]
PLoS Biology, 2018 Large-scale insertional mutagenesis screens can be powerful genome-wide tools if they are streamlined with efficient downstream analysis, which is a serious bottleneck in complex biological systems.
Simon Uhse +5 more
doaj +1 more source
Forward and Reverse Genetics of B Cell Malignancies: From Insertional Mutagenesis to CRISPR-Cas
Frontiers in Immunology, 2021 Cancer genome sequencing has identified dozens of mutations with a putative role in lymphomagenesis and leukemogenesis. Validation of driver mutations responsible for B cell neoplasms is complicated by the volume of mutations worthy of investigation and ...
Joanna C. Dawes +3 more
doaj +1 more source