Results 11 to 20 of about 595 (210)

Mutagenesis by retroviral insertion in chemical mutagen-generated quasi-haploid mammalian cells

BioTechniques, 2007
Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6–8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially ...
Sung O. Kim, Soon-Duck Ha, Sangun Lee, Sarah Stanton, Bruce Beutler, Jiahuai Han   +5 more
doaj   +1 more source

Efficient Transposition of the Retrotransposon Tnt1 in Cucumber (Cucumis sativus L.)

Horticultural Plant Journal, 2018
Tnt1 is an active retrotransposon originally identified in tobacco (Nicotiana tabacum L.) (Grandbastien et al., 1989), but its transposition activity could be activated through tissue culture in other plant species.
Qi ZHANG, Hui DU, Duo LV, Tingting XIAO, Jian PAN, Huanle HE, Gang WANG, Run CAI, Yiqun WENG, Junsong PAN   +9 more
doaj   +1 more source

Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve

Frontiers in Microbiology, 2021
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt, Emily C. Hoedt, Francesca Bottacini, Francesca Bottacini, Nora Cash, Roger S. Bongers, Kees van Limpt, Kaouther Ben Amor, Jan Knol, Jan Knol, John MacSharry, John MacSharry, John MacSharry, Douwe van Sinderen, Douwe van Sinderen   +14 more
doaj   +1 more source

Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

BMC Microbiology, 2018
Background Transposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid.
Santa S. Naorem, Jin Han, Stephanie Y. Zhang, Junyi Zhang, Lindsey B. Graham, Angelou Song, Cameron V. Smith, Fariha Rashid, Huatao Guo   +8 more
doaj   +1 more source

Development of mPing‐based activation tags for crop insertional mutagenesis

Plant Direct, 2021
Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits.
Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A. L. McDaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock   +12 more
doaj   +1 more source

BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage

Nature Communications, 2018
Loss of BRCA2 leads to cancer formation. Here, the authors use an insertional mutagenesis approach and identify a multiprotein complex consisting of BRE, USP7 and CDC25A that can support the survival of BRCA2-deficient cells.
Kajal Biswas, Subha Philip, Aditya Yadav, Betty K. Martin, Sandra Burkett, Vaibhav Singh, Anav Babbar, Susan Lynn North, Suhwan Chang, Shyam K. Sharan   +9 more
doaj   +1 more source

Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis

Molecular Plant-Microbe Interactions, 1998
We have initiated a mutational analysis of pathogenicity in the rice blast fungus, Magnaporthe grisea, in which hygromycin-resistant transformants, most generated by restriction enzyme-mediated integration (REMI), were screened for the ability to infect ...
James A. Sweigard, Anne M. Carroll, Leonard Farrall, Forrest G. Chumley, Barbara Valent   +4 more
doaj   +1 more source

Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish. [PDF]

PLoS ONE, 2015
Enhancer trapping (ET) is a powerful approach to establish tissue- or cell-specific reporters and identify expression patterns of uncharacterized genes. Although a number of enhancer-trapping vectors have been developed and a large library of fish lines ...
Chunyan Liu, Guili Song, Lin Mao, Yong Long, Qing Li, Zongbin Cui   +5 more
doaj   +1 more source

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Plant Methods, 2017
Background Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants
Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman, James V. Moroney   +6 more
doaj   +1 more source

Safe engineering of CAR T cells for adoptive cell therapy of cancer using long‐term episomal gene transfer

EMBO Molecular Medicine, 2016
Chimeric antigen receptor (CAR) T‐cell therapy is a new successful treatment for refractory B‐cell leukemia. Successful therapeutic outcome depends on long‐term expression of CAR transgene in T cells, which is achieved by delivering transgene using ...
Chuan Jin, Grammatiki Fotaki, Mohanraj Ramachandran, Berith Nilsson, Magnus Essand, Di Yu   +5 more
doaj   +1 more source