Results 91 to 100 of about 45,101 (293)

A Unifying Scenario on the Origin and Evolution of Cellular and Viral Domains [PDF]

, 2009
The cellular theory on the nature of life has been one of the first major advancements in biology. Viruses, however, are the most abundant life forms, and their exclusion from mainstream biology and the Tree of Life (TOL) is a major paradox in biology ...
Claudiu I. Bandea
core   +1 more source

ATIDB: Arabidopsis thaliana insertion database [PDF]

, 2003
Insertional mutagenesis techniques, including transposon- and T-DNA-mediated mutagenesis, are key resources for systematic identification of gene function in the model plant species Arabidopsis thaliana.
Pan, X. K., Liu, H., Clarke, J., Jones, J., Bevan, M., Stein, L.   +5 more
core   +1 more source

The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NO3− Transporter ZmCLCa

Advanced Science, EarlyView.
In maize, the bHLH transcription factor ZmbHLH118 directly binds to the promoter of ZmCLCa and inhibits its expression. Tonoplast‐localized ZmCLCa mediates NO3− influx into the vacuole to regulate intracellular NO3− homeostasis, modulating nitrate uptake and metabolism, plant growth, and grain yield.
Chaonan Zhang, Elsa Demes‐Causse, Xujian Li, Zhenhui Guo, Hanshu Zhao, Huairong Cao, Yajing Song, Lijun Mu, Kaikai Zhang, Jing Zhang, Zhongtao Jia, Lixing Yuan, Alexis De Angeli, Jingbo Zhang   +13 more
wiley   +1 more source

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit

Nature Communications
Site-directed insertion is a powerful approach for generating mutant alleles, but low efficiency and the need for customisation for each target has limited its application.
Lee B. Miles, Vanessa Calcinotto, Sara Oveissi, Rita J. Serrano, Carmen Sonntag, Orlen Mulia, Clara Lee, Robert J. Bryson-Richardson   +7 more
doaj   +1 more source

International Consortium of Rice Mutagenesis: Resources and beyond [PDF]

, 2013
Rice is one of the most important crops in the world. The rice community needs to cooperate and share efforts and resources so that we can understand the functions of rice genes, especially those with a role in important agronomical traits, for ...
Droc, Gaëtan, Guiderdoni, Emmanuel, Hsing, Yue-ie C., Wei, Fu-Jin   +3 more
core   +2 more sources

Transposon Insertion Mutagenesis for Archaeal Gene Discovery [PDF]

, 2016
Archaea constitute the third domain of life, but studies on their physiology and other features have lagged behind bacteria and eukarya, largely due to the challenging biology of archaea and concomitant difficulties in methods development. The use of genome-wide en masse insertion mutagenesis is one of the most efficient means to discover the genes ...
Kiljunen, Saija Johanna, Pajunen, Maria Irene, Savilahti, Harri Tapani   +2 more
openaire   +3 more sources

Systematically Engineering for Efficient Production of 3‐Methyl‐1‐Butanol in Escherichia coli

Advanced Science, EarlyView.
An integrated metabolic engineering strategy was established for high‐level 3‐methyl‐1‐butanol biosynthesis in Escherichia coli. Molecular dynamics‐guided semi‐rational engineering of dihydroxyacid dehydratase uncovered and relieved key catalytic bottlenecks, while adaptive laboratory evolution enhanced strain robustness.
Nanfei Geng, Hao Liu, Haolin Han, Meng Meng, Shaojie Wang, Tianwei Tan, Haijia Su   +6 more
wiley   +1 more source

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source

Deciphering the Evolution Pattern of Structural Variations Overlapped With Repetitive Sequence During Cattle Evolution

Advanced Science, EarlyView.
The authors complement bovine pan‐SV with massive novel structural variations (SVs) identified through long‐read sequencing of 83 globally distributed cattle breeds. Repetitive sequence‐mediated SVs (rep‐SV) exhibit distinct dynamic patterns throughout cattle sub‐speciation and/or domestication processes, including uneven distribution between chr‐X and
Zhifan Guo, Jinxiu Li, Adeniyi C. Adeola, Xueyan Jiang, Juntao Ma, Jian Xiao, Dexiang Hu, Kaixing Qu, Haihong Wu, Junren Chen, Zhanxing He, Tingting Yin, Ali Esmailizadeh, Jing Luo, Olivier Hanotte, Ya‐Ping Zhang, Yan Li   +16 more
wiley   +1 more source

Retrotransposon Tto1: functional analysis and engineering for insertional mutagenesis [PDF]

, 2011
Retrotransposons are genomic parasites activated by stress conditions that can be seriously detrimental for their host. In this work I demonstrate that Tto1, a typical plant LTR retrotransposon with insertion preference into genes can be turned into a ...
Tramontano, Andrea, Tramontano, Andrea, Tramontano, Andrea   +2 more
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