Results 101 to 110 of about 45,101 (293)

Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants [PDF]

, 2011
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification ...
Catalanotti, Claudia, Fernández Reyes, Emilio, Galván Cejudo, Aurora, González-Ballester, David, Grossman, Arthur R., Higuera, José J., Magneschi, Leonardo, Montaigu, Amaury de, Mus, Florence, Pootakham, Wirulda, Prior, Matthew, Yang, Wenqiang   +11 more
core   +2 more sources

Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System

Advanced Science, EarlyView.
Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei, Wei Liu, Bili Cao, Dan Zhao, Chengqiang Wang, Xin Hou, Tangyuan Ning, Biao Gong   +7 more
wiley   +1 more source

Sleeping Beauty Mouse Models of Cancer: Microenvironmental Influences on Cancer Genetics

Frontiers in Oncology, 2019
The Sleeping Beauty (SB) transposon insertional mutagenesis system offers a streamlined approach to identify genetic drivers of cancer. With a relatively random insertion profile, SB is uniquely positioned for conducting unbiased forward genetic screens.
Amy Guimaraes-Young, Charlotte R. Feddersen, Adam J. Dupuy   +2 more
doaj   +1 more source

In Vivo RNAi Screening Identifies Regulators of Actin Dynamics as Key Determinants of Lymphoma Progression [PDF]

, 2009
April 1, 2010Mouse models have markedly improved our understanding of cancer development and tumor biology. However, these models have shown limited efficacy as tractable systems for unbiased genetic experimentation.
A Besson, A Leask, AG Uren, AJ Dupuy, AJ Ridley, B Luo, Corbin E Meacham, DA Grueneberg, DJ Burgess, E Helfer, Emily E Ho, Esther Dubrovsky, Frank B Gertler, H Nishihara, J Jonkers, JA Cancelas, JA Nick, JM Adams, K Berns, K Burridge, L Zender, LG Smith, LS Collier, M Margulies, M van Lohuizen, Michael T Hemann, MR Schlabach, MT Hemann, N Tani, PN Kelly, R Firestein, RA Dickins, S Kleber, T Suzuki, TF Westbrook, VN Ngo, Y Gao   +36 more
core   +3 more sources

Machine Learning‐Enhanced Random Matrix Theory Design for Human Immunodeficiency Virus Vaccine Development

Advanced Intelligent Discovery, EarlyView.
This study integrates random matrix theory (RMT) and principal component analysis (PCA) to improve the identification of correlated regions in HIV protein sequences for vaccine design. PCA validation enhances the reliability of RMT‐derived correlations, particularly in small‐sample, high‐dimensional datasets, enabling more accurate detection of ...
Mariyam Siddiqah, Muhammad Zulqarnain Zeb, Muhammad Husnain Zeb, Babar Shabbir   +3 more
wiley   +1 more source

Analysis of tumor heterogeneity and cancer gene networks using deep sequencing of MMTV-induced mouse mammary tumors.

PLoS ONE, 2013
Cancer develops through a multistep process in which normal cells progress to malignant tumors via the evolution of their genomes as a result of the acquisition of mutations in cancer driver genes. The number, identity and mode of action of cancer driver
Christiaan Klijn, Marco J Koudijs, Jaap Kool, Jelle ten Hoeve, Mandy Boer, Joost de Moes, Waseem Akhtar, Martine van Miltenburg, Annabel Vendel-Zwaagstra, Marcel J T Reinders, David J Adams, Maarten van Lohuizen, John Hilkens, Lodewyk F A Wessels, Jos Jonkers   +14 more
doaj   +1 more source

A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites

, 2009
Insertional mutagens such as viruses and transposons are a useful tool for performing forward genetic screens in mice to discover cancer genes. These screens are most effective when performed using hundreds of mice, however until recently a major ...
A Ambrosi, AG Uren, AJ Dupuy, AM Bender, Anders H Lund, Anthony G Uren, Anton Berns, C Greenman, Catherine H Wilson, David J Adams, EK Hui, FA Giordano, GP Wang, H Mikkers, H Mikkers, H Ochman, Harald Mikkers, HT Cuypers, J Kong, Jaap Kool, JC Mullikin, Jos Jonkers, Louise van der Weyden, LS Collier, M Margulies, MA Marra, MA McAleer, Maarten van Lohuizen, OS Kustikova, P Parameswaran, PN Hengen, Richard Rance, RS Devon, VW Keng, X Wu   +34 more
core   +1 more source

Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. [PDF]

, 2017
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost
ARAÚJO, W. L. de, FAVARO, L. C. de L., FERREIRA, A., PALLU, A. P. de S., PIZZIRANI-KLEINER, A. A., RODRIGUES, M. B. C., RODRIGUES, M. J. C., SEBASTIANES, F. de S., SPÓSITO, M. B.   +8 more
core   +1 more source

AI Guided Protein Design for Next‐Generation Autogenic Engineered Living Materials

Advanced Intelligent Systems, EarlyView.
Autogenic engineered living materials (ELMs) integrate biology and materials science to create self‐regenerating and self‐healing materials. This perspective highlights emerging strategies in protein engineering and AI‐guided de novo design to expand the capabilities of autogenic ELMs.
Hoda M. Hammad, Anna M. Duraj‐Thatte
wiley   +1 more source

Zebrafish and CRISPR—A synergistic approach to decipher and cure human diseases

Animal Models and Experimental Medicine, EarlyView.
Zebrafish, with high genetic homology to humans, serves as a powerful vertebrate model for disease modeling and drug discovery. Integration of CRISPR/Cas9 technology enables precise genome editing, facilitating the development of translational models for human diseases.
Manikandan Sivaprakasam, Aashika Raagavi Jeanpierre, Salma Mohammed, Rajesh Srinivasan, Aman Kumar Mohanty   +4 more
wiley   +1 more source