Results 131 to 140 of about 595 (210)

Systemic and Local Regulation of Root Growth by Vascular Trehalose 6‐Phosphate is Correlated With Re‐allocation of Primary Metabolites Between Shoots and Roots

Plant, Cell &Environment, EarlyView.
ABSTRACT Trehalose 6‐phoshpate (Tre6P) is a key signalling molecule that reflects carbon status and integrates it with developmental decision making. Tre6P has been demonstrated to regulate various developmental processes, including vegetative growth, shoot branching, flowering, and root branching.
Moritz Göbel, Jacqueline Foster, Philipp Westhoff, Noemi Skorzinski, Hannah L. Lepper, Maria F. Njo, Markus Schmid, Tom Beeckman, Miloš Tanurdžić, Anna Amtmann, Franziska Fichtner   +10 more
wiley   +1 more source

Conjugal vectors for cloning, expression, and insertional mutagenesis in Gram-negative bacteria

BioTechniques, 2005
Federico M. Lauro, Emiley A. Eloe, Nicoletta Liverani, Giulio Bertoloni, Douglas H. Bartlett   +4 more
doaj   +1 more source

Caenorhabditis elegans as an in vivo model system for human inherited primary arrhythmia syndromes

The Journal of Physiology, EarlyView.
Abstract figure legend Most genes involved in inherited primary arrhythmia syndromes (IPAS) are conserved in Caenorhabditis elegans, where genetic manipulation enables functional characterization of variants, identification of regulatory proteins, and in vivo drug testing.
Antoine Delinière, Thomas Boulin, Maëlle Jospin, Alexandre Janin, Gilles Millat, Philippe Chevalier, Olga Andrini   +6 more
wiley   +1 more source

Technologies for engineering repetitive DNA

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract Repetitive DNA, a fundamental architectural element of genomes, is widespread across organisms and comprises about 54% of the human genome. With advances in long‐read sequencing and bioinformatics approaches, highly repetitive sequences can now be characterized in depth.
Shuting Ma, Yali Cui, Yi Wu
wiley   +1 more source

Magnesium as a conformational gatekeeper of KRAS: Structural dynamics and therapeutic implications

Protein Science, Volume 35, Issue 7, July 2026.
Abstract Magnesium serves as an essential cofactor for small GTPases, yet its structural role in regulating KRAS conformational dynamics and nucleotide exchange remains poorly understood. Here, we combine hydrogen–deuterium exchange mass spectrometry (HDX‐MS), native mass spectrometry, and functional assays to elucidate how Mg2+ stabilizes the KRAS ...
Bindu Y. Srinivasu, Tanvi S. Damerla, Alexander Stec, Zhiwei Zhou, John R. Engen, Kenneth D. Westover, Thomas E. Wales   +6 more
wiley   +1 more source

The L108I polymorphism in mouse prion protein drives spontaneous disease and enhances transmission of atypical and classical prion strains

Brain Pathology, Volume 36, Issue 4, July 2026.
A single amino acid change (L108I) combined with PrP overexpression drives spontaneous atypical prion formation in mice, enabling also efficient propagation of diverse prion strains. This model allows studying how spontaneous prion diseases arise and provides powerful tools for investigating strain emergence, transmission barriers, and mechanisms ...
Hasier Eraña, Enric Vidal, Natalia Fernández‐Borges, Jorge M. Charco, Carlos M. Díaz‐Domínguez, Cristina Sampedro‐Torres‐Quevedo, Josu Galarza‐Ahumada, Eva Fernández‐Muñoz, Maitena San‐Juan‐Ansoleaga, Miguel Ángel Pérez‐Castro, Nuno Gonçalves‐Anjo, Patricia Piñeiro, Samanta Giler, Nora González‐Martín, Nuria L. Lorenzo, Africa Manero‐Azua, Guiomar Perez de Nanclares, Mariví Geijo, Manuel A. Sánchez‐Martín, Jesús R. Requena, Joaquín Castilla   +20 more
wiley   +1 more source

The amino terminal domain of the human α7 nicotinic acetylcholine receptor subunit leads to the functional expression of human/insect receptors

British Journal of Pharmacology, Volume 183, Issue 13, Page 3517-3537, July 2026.
Human α7 receptors have been characterised in terms of pharmacological properties. Insertion of the N‐terminal domain of the human α7 subunit leads to honeybee and cockroach chimeric receptors activated by ACh and inhibited by α‐Bgt. Insertion of the human cys‐loop leads to cockroach chimeric receptors modulated by PNU.
Alison Cartereau, Chloé Veront, Alexandra Cremades, Priscilla Martin, Remi Gojard, Nicolas Galland, Emiliane Taillebois, Dieudonnée Togbé, Jean‐Yves Le Questel, Steeve H. Thany   +9 more
wiley   +1 more source

Gene Therapy and Gene Editing in Type 1 Diabetes: CRISPR‐Based β‐Cell Replacement and Treg Immune Modulation Approaches

Diabetes, Obesity and Metabolism, Volume 28, Issue 7, Page 5476-5491, July 2026.
ABSTRACT Background Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease marked by the destruction of pancreatic β‐cells, resulting in lifelong dependence on exogenous insulin. Despite advances in insulin delivery and glucose monitoring technologies, patients remain at risk for acute and long‐term complications, underscoring the need for ...
Tzu‐Min Lin, Hui‐Fang Chang, Tzu‐Ching Lin, Cheng‐Han Lin, Yu‐Ling Sun, Chih‐Sheng Lin   +5 more
wiley   +1 more source

The Transcription Factor OsWRKY64 Interacts With OsART1 to Positively Regulate Al Resistance in Rice

Plant Biotechnology Journal, Volume 24, Issue 7, Page 4656-4671, July 2026.
A proposed working model illustrating the cooperative regulation of Al tolerance by OsWRKY64 and OsART1 in rice. Under Al stress conditions in acidic soil, the transcription factor OsART1 directly binds to the promoter of OsWRKY64 to activate its transcription. The synthesized OsWRKY64 protein then physically interacts with OsART1 in the nucleus.
Changzhao Chen, Qiang Zhang, Yonggang Xu, Jibo Yang, Jing Yan, Mengmeng Jiang, Lu Zheng, Tingwu Liu, Jiu Huang, Renfang Shen, Xiaofang Zhu   +10 more
wiley   +1 more source

Single‐Cell CRISPR: An Efficient Strategy for Decoding Plant Cis‐Regulatory Complexity

Plant Biotechnology Journal, Volume 24, Issue 7, Page 4454-4467, July 2026.
ABSTRACT The generation of complex traits involves the coordinated interplay of multiple gene networks. Elucidating the function of transcriptional cis‐regulatory elements (CREs) in regulating gene expression is crucial for understanding complex regulatory pathways and improving our ability to modify macro‐phenotypes.
Yuanhang Zhang, Xuanxuan Luo, Hongbin Li, Shuangxia Jin, Xianlong Zhang, Maojun Wang   +5 more
wiley   +1 more source