Results 141 to 150 of about 595 (210)

Prime Editing of Phytoene Synthase 1 in Rice for Seed Carotenoid Biofortification

Plant Biotechnology Journal, EarlyView.
Byeong‐Hoon Kim, Yeo Jin Lee, Yong Jin Choi, Sooyeon Lim, Young Jin Park, Jae Kwang Kim, Sun‐Hwa Ha   +6 more
wiley   +1 more source

Field Trials and Baking Studies of Ultra‐Low Asparagine, Genome Edited (CRISPR/Cas9) and Mutant (TILLING) Wheat

Plant Biotechnology Journal, Volume 24, Issue 7, Page 4704-4715, July 2026.
ABSTRACT Field trials were conducted of wheat (Triticum aestivum) cv. Cadenza in which asparagine synthetase gene, TaASN2, had been knocked out, either on its own or together with a partial knockout of the related gene, TaASN1, using CRISPR/Cas9. Chemical mutagenesis (TILLING) TaASN2 nulls in the Claire background were also included.
Navneet Kaur, Sarah Raffan, Suzanne J. Clark, Shpresa Musa, Katharina Scherf, J. Stephen Elmore, Tanya Y. Curtis, Emma Honan, Nigel G. Halford   +8 more
wiley   +1 more source

CRISPR/Cas‐Mediated Gene Editing in Plant Immunity and Its Potential for the Future Development of Fungal, Oomycete, and Bacterial Pathogen‐Resistant Pulse Crops

Plant, Cell &Environment, Volume 49, Issue 7, Page 3573-3585, July 2026.
ABSTRACT Pulses provide myriad health benefits and are advantageous in an environmental context as a result of their leguminous nature. However, phytopathogenic fungi, oomycetes and bacteria pose a substantial threat to pulse production, at times leading to crop failure.
Stacy D. Singer, Mohammed M. Mukthar, Udaya Subedi, Hari Poudel, Guanqun Chen, Nora Foroud, Syama Chatterton   +6 more
wiley   +1 more source

Mutagenic Survey of Key Residues of NifB Involved in Radical SAM‐Dependent Nitrogenase Cofactor Assembly

ChemBioChem, Volume 27, Issue 11, 15 June 2026.
NifB uses an RS–K1–K2 triad to assemble the L‐cluster, a [Fe8S9C] precursor of the nitrogenase cofactor. The RS module cleaves SAM to form the 5′‐dA radical, K1 serves structural/SAM‐sensing roles, and K2 drives radical‐dependent fusion with K1. SAM‐anchoring residues link SAM binding to cluster fusion, while termini and surface residues regulate ...
Calder Duffin, Robert Quechol, Yimo Yang, Chi Chung Lee, Yilin Hu, Markus W. Ribbe   +5 more
wiley   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, Volume 16, Issue 6, Page 1132-1141, June 2026.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Temporal and Cell‐Specific Regulation of Synaptic Homeostasis by the Chromatin Remodeler Chd1

Advanced Science, Volume 13, Issue 33, 15 June 2026.
Chd1, the Drosophila homologue of mammalian CHD2 ‐ a gene linked to autism, epilepsy, and intellectual disability, is required for synaptic homeostatic plasticity. Chd1 in glia is necessary for the rapid induction of synaptic homeostasis, whereas Chd1 in motoneurons, muscle, and glia is critical for long‐term maintenance.
Danielle T. Morency, Tao Cui, Yimei Cai, Chloe Lok, Rachel E. Nokku, Ruoxian Huang, Grace L. Chu, Yumeng Xie, Saleem W. Abu‐Tayeh, Kaikai He, Chengjie Qiu, Junyi Wang, Paxton M. Paganelli, Ting Wang, Gabrielle Williams, Sreejith Nair, Huadong Pei, Dion K. Dickman, Stefano Vicini, Tingting Wang   +19 more
wiley   +1 more source

Connexin 43‐Enriched Vesicles Improve Synchronization in hiPSC‐Derived Cardiomyocytes

Advanced Science, Volume 13, Issue 33, 15 June 2026.
Plasma membrane‐derived Connectosomes enriched in connexin‐43 are produced from donor hiPSCs and applied to hiPSC‐derived cardiomyocytes (hiPSC‐CMs). Connexin‐43 channels on Connectosomes remain functional, strengthening intercellular electrochemical coupling and increasing network synchronization over time.
Nima Momtahan, Anna K. McClain, Andrea Trementozzi, Sogu Sohn, Lingxia Qiao, Chi Zhao, Padmini Rangamani, Jeanne C. Stachowiak, Janet Zoldan   +8 more
wiley   +1 more source

Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System

Advanced Science, Volume 13, Issue 32, 9 June 2026.
Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei, Wei Liu, Bili Cao, Dan Zhao, Chengqiang Wang, Xin Hou, Tangyuan Ning, Biao Gong   +7 more
wiley   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, Volume 13, Issue 32, 9 June 2026.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

The Development and Characterization of a Panel of Amine Dehydrogenases (AmDHs) and Amino Acid Dehydrogenases (AADHs)

ChemCatChem, Volume 18, Issue 11, 15 June 2026.
The CoE‐AmDH panel enables sustainable access to chiral amines using diverse amine (AmDH) and amino acid dehydrogenases (AADH). Utilizing formate (FDH) or glucose dehydrogenase (GDH) for cofactor regeneration, this study establishes a robust screening platform for mild reductive aminations.
R. Tilly Bradshaw Allen, William R. Birmingham, Nicholas John Turner   +2 more
wiley   +1 more source