Results 161 to 170 of about 595 (210)

Insertional mutagenesis in transgenic mice

Transgenic Research, 1994
Increasing numbers of transgenic mouse lines have resulted in several dozens of mutants created by insertional mutagenesis. The advantages of different vector systems and the problems associated with the analysis of mutations and the cloning of the affected genes are discussed in this review.
T, Rijkers, A, Peetz, U, Rüther
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Insertion mutagenesis of Escherichiacoli GroEL

Biochemical and Biophysical Research Communications, 2003
To gain insights into the in vivo folding and assembly of bacterial chaperonins, groEL was subjected to insertion mutagenesis using transposon ISlacZ/in. Four GroEL-LacZ fusions and the corresponding insertion mutants were obtained after residues 34, 90, 291, and 367. Apical domain insertion mutants GroEL291 and GroEL367 were degraded into monomeric 30-
Danielle, Amatore, François, Baneyx
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Transposon Insertional Mutagenesis in Rice

2010
Insertion mutants offer one of the direct ways to relate a gene to its function by employing forward or reverse genetics approaches. Both T-DNA and transposon insertional mutants are being produced in several crops, including rice, the first cereal with its complete genome sequenced.
Narayana M, Upadhyaya, Qian-Hao, Zhu, Ramesh S, Bhat   +2 more
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Insertional mutagenesis in mice

Trends in Genetics, 1987
Abstract Insertional mutagenesis via the introduction of exogenous DNA into the germ line has been accomplished in many organisms. In addition to causing the mutation, the introduced DNA serves as a tag for molecular cloning of the affected locus. A number of mutations due to the insertion of either retroviral or microinjected cloned DNA have been ...
Thomas Gridley, Philippe Soriano, Rudolf Jaenisch   +2 more
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T-DNA insertion mutagenesis.

2012
This chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also
Sangwan, Rajbir S., Ochatt, Sergio, Nava-Saucedo, José-Edmundo, Sangwan-Norreel, Brigitte   +3 more
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Retroviral-Mediated Insertional Mutagenesis in Zebrafish

2004
Publisher Summary This chapter discusses retroviral-mediated insertional mutagenesis in zebrafish. Large-scale chemical mutagenesis screens have resulted in the isolation of thousands of mutations in hundreds of genes that affect zebrafish embryonic development.
A, Amsterdam, N, Hopkins
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T-DNA insertional mutagenesis in Arabidopsis

Plant Molecular Biology, 1992
T-DNA insertion mutagenesis is one of those recently developed genetic techniques which can be expected to have a major impact in plant molecular biology [17, 50, 87]. Unlike conventional reviews, this brief outlook intends to give a practical insight into current problems and future applications of T-DNA tagging by underlining the potential of the ...
C, Koncz, K, Németh, G P, Rédei, J, Schell   +3 more
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Gene Therapy Insertional Mutagenesis Insights

Science, 2004
In a milestone study describing the first “cure” of a genetic disease by retroviral gene therapy, 9 out of 10 infants born with X-linked severe combined immunodeficiency (SCID-X1, also known as γc or IL2RG deficiency) were successfully treated with autologous bone marrow stem cells infected ...
Utpal P, Davé, Nancy A, Jenkins, Neal G, Copeland   +2 more
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Efficient insertional mutagenesis in Streptococcus thermophilus

Gene, 2000
Bacteria have always been considered ideal organisms for genetic analysis. While this is true for some model organisms, like Escherichia coli, Bacillus subtilis and, more recently, Lactococcus lactis, genetic analysis of other organisms is often prevented by lack of valuable tools, like vectors, transposons and methods for transformation, gene ...
BACCIGALUPI L., NACLERIO, Gino, DE FELICE M., RICCA E.   +3 more
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Ty insertional mutagenesis

1992
Abstract The use of transposable elements as insertional mutagens is an extremely powerful technology for genetic analysis. This approach was brought to a fine art in prokaryotic genetics and is proving equally applicable in fruit flies, mice, and higher plants.
J N Strathern, M Mastrangelo, L A Rinckel,, D J Garfinkel   +3 more
openaire   +1 more source