Results 231 to 240 of about 45,101 (293)

UPO‐Mimetic Engineering of CYP199A4 Enables Enhanced Peroxygenase Activity via the Hydrogen Peroxide Shunt Pathway

ChemistryEurope, Volume 4, Issue 3, March 2026.
P450 goes peroxide: When one base just isn’t basic enough. Engineering a second basic residue into CYP199A4T252E boosts its peroxygenase activity but also makes it sensitive to H2O2. The double mutant F182R/T252E shows increased turnover before rapidly self‐destructing, revealing the fine balance between productive peroxide activation and oxidative ...
Pablo Velázquez García, Thomas Hilberath, Peter‐Leon Hagedoorn, Frank Hollmann   +3 more
wiley   +1 more source

CRISPR Assays for Protein Detection

Chemistry–Methods, Volume 6, Issue 3, March 2026.
This review summarizes recent advances in CRISPR–Cas–based strategies for protein detection. Particular focus is placed on recognition element–based approaches, in which antibodies, aptamers, or DNAzymes convert protein binding into CRISPR‐readable nucleic acid signals, and activity‐based strategies that exploit intrinsic protein functions to trigger ...
Luca Capelli, Elena Spezzani, Dharshini Raghavan, Chiara Micucci, Alessandra Zanut, Alessandro Bertucci   +5 more
wiley   +1 more source

Exosome‐Loaded Engineered circBDNF Promotes Spinal Cord Injury Repair Through the PI3K/AKT/mTOR Signaling Axis

CNS Neuroscience &Therapeutics, Volume 32, Issue 3, March 2026.
Engineered exosomes carrying circBDNF promote spinal cord injury repair by activating the PI3K/AKT/mTOR pathway. This treatment reduces apoptosis and inflammation, enhances axonal regeneration, and restores motor function, offering a promising neurotrophic strategy for spinal cord repair. ABSTRACT Background The brain‐derived neurotrophic factor (BDNF)
Gang Li, Ganggang Kong, Cheng Gu, Di Zhang, Yong Wan, Baoshu Xie   +5 more
wiley   +1 more source

c‐MAF Transcriptionally Activates Slc40a1 to Repress Ferroptosis in Sepsis‐Associated Encephalopathy

CNS Neuroscience &Therapeutics, Volume 32, Issue 3, March 2026.
c‐MAF and its downstream target Slc40a1 were identified as key regulators of ferroptosis in septic mouse hippocampi. Neuron‐specific knockdown of either gene aggravated CLP‐induced cognitive deficits and ferroptosis, whereas their overexpression exerted protective effects.
Wenqin Song, Qianni Shen, Hui Zhang, Xueshan Bu, Wenwei Gao, Wei Wang   +5 more
wiley   +1 more source

Transposable elements: Functional aspects and applications as drivers of crop innovation

Crop Science, Volume 66, Issue 2, March/April 2026.
Abstract Transposable elements (TEs), which were once relegated to the status of genomic parasites, are today accepted as important agents of plant genome evolution and adaptation. This review integrates evidence pointing to their dual function as drivers of genetic diversity and instability, with a focus on their contribution to phenotypic innovation,
Flávia Layse Belém Medeiros, José Ribamar Costa Ferreira‐Neto, Jarbson Henrique Oliveira Silva, Cinthia Carla Claudino Grangeiro Nunes, Ana Luíza Trajano Mangueira de Melo, Ayug Bezerra Lemos, Madson Allan de Luna‐Aragão, Agnes Angélica Guedes de Barros, Simon Orozco‐Arias, Eliseu Binneck, Francismar Correa Marcelino‐Guimarães, Ana Cristina Brasileiro‐Vidal, Ana Maria Benko‐Iseppon   +12 more
wiley   +1 more source

Ninjurin1 in cardiovascular and vascular biology: From molecular mechanisms to therapeutic opportunities

Clinical and Translational Medicine, Volume 16, Issue 3, March 2026.
NINJ1 mediates PMR in cardiovascular cell death, releasing DAMPs that amplify vascular inflammation. Dual NINJ1 paradox: membrane‐bound form drives endothelial NF‐κB/CXCL‐8 inflammation while soluble form provides atheroprotection. Pericyte NINJ1 regulates the critical balance between angiogenic sprouting and vessel maturation in ischaemic tissues ...
Muhammad Mamunur Rashid Mahib
wiley   +1 more source

Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato. [PDF]

PLoS One
Kumari A, Ponukumatla R, Pandey AK, Sreelakshmi Y, Sharma R.   +4 more
europepmc   +1 more source

Gene-edited hematopoietic stem cells for leukemia and lymphoma treatment: a systematic review of preclinical and translational evidence. [PDF]

Discov Oncol
Nourmohammadi H, Babashahi M, Panji M, Radmehr S.   +3 more
europepmc   +1 more source

An improved lentivirus vector for safer transgene integration and protein transduction [PDF]

Schenkwein, Diana
core  

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T-DNA insertion mutagenesis.

2012
This chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also
Sangwan, Rajbir S., Ochatt, Sergio, Nava-Saucedo, José-Edmundo, Sangwan-Norreel, Brigitte   +3 more
openaire   +2 more sources