Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Distribution of follow-up sessions influences determinations of skill maintenance. [PDF]
Mutchler MK, Pence ST.
europepmc +1 more source
Enhancing the Matrix Transpose Operation Using Intel Avx Instruction Set Extension
Ahmed Zekri
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Validating and prioritizing key indicators for blended MOOC implementation in English language learning using the fuzzy Delphi method. [PDF]
Pham AT, Abdullah MRTL.
europepmc +1 more source
A Practical Quantum Instruction Set Architecture
Robert Smith +2 more
openalex +2 more sources
Exam‐level analysis of lecture capture viewing and student exam performance
This study asks how lecture capture relates to exam performance in biology courses with three noncumulative exams. Mixed‐effects modeling separated between‐students from within‐student effects on exam %. Students who watched more recordings earned higher grades, but viewing spikes above a student's own mean, consistent with cramming, predicted small ...
Kirk Hillsley
wiley +1 more source
Construction of online classroom instructional quality assessment system of university music based on BP neural network. [PDF]
Ouyang R.
europepmc +1 more source
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source
Optimizing Scanning in Youth Female Football: The Role of Verbal Instructions. [PDF]
Hintermann M +4 more
europepmc +1 more source

