Results 11 to 20 of about 28,566 (150)

PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens. [PDF]

, 2017
Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy ...
Bath, Tyler, Esko, Jeffrey D, Harismendy, Olivier, Kim, Jihoon, Lewis, Nathan E, Spahn, Philipp N, Weiss, Ryan J   +6 more
core   +2 more sources

Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 [PDF]

, 2011
The 5'-cap-structures of higher eukaryote mRNAs are ribose 2'-O-methylated. Likewise, a number of viruses replicating in the cytoplasm of eukayotes have evolved 2'-O-methyltransferases to modify autonomously their mRNAs.
A Ghosh, A Nomoto, A Pichlmair, AM Waterhouse, B Morin, Benjamin W Neuman, BS Schnierle, Burkhard Ludewig, CA Janeway Jr., DJ Hui, E Decroly, EJ Snijder, F Terenzi, G Bocharov, G Uzé, H Hemmi, H Kato, H Zhou, J Reguera, JK Roth-Cross, John Ziebuhr, K Bryson, K Karikó, KK Eriksson, KM Rose, Kristy J Szretter, L Cervantes-Barragan, L Cervantes-Barragán, L Gitlin, Luisa Cervantes-Barragan, M Bouvet, M Feder, M Schlee, Matthias Habjan, Michael S Diamond, O Haller, O Le Gall, O Takeuchi, R Gosert, R Züst, Reinhard Maier, Roland Züst, S Daffis, S Shuman, S Wünschmann, SR Nallagatla, SR Nallagatla, Stuart G Siddell, Susan C Baker, V Hornung, V Thiel, Volker Thiel, Winfried Barchet, Y Chen, YM Loo, YM Loo   +55 more
core   +1 more source

Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures

, 2019
Here, using an integrative experimental and computational approach, Imle et al. show how cell motility and density affect HIV cell-associated transmission in a three-dimensional tissue-like culture system of CD4+ T cells and collagen, and how different ...
Ales, J., Carrillo-Bustamante, P., Fackler, O., Fehr, J., Godinez, W., Graw, F., Hamprecht, F., Imle, A., Kumberger, P., Müller, B., Ritter, C., Rohr, K., Schmidt, P., Schnellbächer, N., Schwarz, U.   +14 more
core   +2 more sources

An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J, Chan, Yingleong, Chavez, Alejandro, Church, George M, Church, George M, Collins, James J, Davidsohn, Noah, Ebrahimkhani, Mo R, Guo, Xiaoge, Kiani, Samira, Kuo, Chih-Chung, Lance-Byrne, Alissa, Lewis, Nathan E, Lim, Elaine T, Menn, David, Milanova, Denitsa, Pradhan, Swechchha, Sharma, Sumana, Tung, Angela, Tuttle, Marcelle, Yeo, Nan Cher   +20 more
core   +1 more source

Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. [PDF]

, 1985
Three monoclonal antibodies that react with previously undefined antigenic determinants on the clathrin molecule have been produced and characterized. They were isolated from a fusion between myeloma cells and popliteal lymphocytes from SJL mice that had
Brodsky, Frances
core  

Report on the 2012 Proficiency Test on pyrrolizidine alkaloids in honey and hay [PDF]

, 2013
The purpose of this proficiency test was to investigate the current measurement capacities of testing laboratories for pyrrolizidine alkaloids in honey and plant materials.
MISCHKE Carsten, MULDER Patrick P J, STROKA Joerg, TAMOSIUNAS VYTAUTAS   +3 more
core   +1 more source

PEDOT‐Based Eutectogel Electrode Arrays for Enhanced High‐Resolution Electrogastrography: Fabrication, Stability, and Wearable Performance

Advanced Functional Materials, EarlyView.
Here, we present a high‐density PEDOT eutectogel electrode array for enhanced body surface gastric mapping. Silver electrodes are blade‐coated onto flexible substrates, followed by electrogelation of PEDOT:PSS and the deposition of a PEDOT:LS eutectogel.
Christopher Slaughter, Luke Gatecliff, Xudong Tao, Ruben Ruiz‐Mateos Serrano, Amy T. Jin, Matias L. Picchio, Daniele Mantione, Antonio Dominguez‐Alfaro, George G. Malliaras   +8 more
wiley   +1 more source

Team-oriented process programming [PDF]

, 1991
Team-oriented process programming promises to provide significant support for the planning, directing, and controlling of software engineering projects.
Taylor, Richard N., Young, Patrick S.
core  

Multifunctional Bio‐Based Packaging for Perishable Foods: Structural Design, Scalable Fabrication, and Versatile Applications

Advanced Materials, EarlyView.
An overview of design principles and scalable fabrication strategies for multifunctional bio‐based packaging. Radiative cooling films, modified‐atmosphere films/membranes, active antimicrobial/antioxidant platforms, intelligent optical/electrochemical labels, and superhydrophobic surfaces are co‐engineered from material chemistry to mesoscale structure
Lei Zhang, Jiaqi Zhang, Mei Cui, Wei Qi, Renliang Huang, Rongxin Su, Kai Zhang   +6 more
wiley   +1 more source

Organ‐Specific and Conserved Regulatory Logic Orchestrates Gene Expression in the Embryonic Mesothelium

Advanced Science, EarlyView.
Integrated multi‐omic profiling maps the gene‐regulatory landscape of the coelomic mesothelium across heart, lung, and pancreas. A cardiac‐restricted regulatory program is uncovered in which TBX20 activates heart mesothelial (epicardial) cis‐regulatory elements, while MAF emerges as a conserved regulator of mesothelial identity.
Quang Minh Dang, Nicola Smart, Andia Nicole Redpath, Joaquim Miguel Vieira   +3 more
wiley   +1 more source