Results 121 to 130 of about 199,615 (291)
EBioMedicine, 2017 Background: Recently, it was demonstrated that proteins can be translated from alternative open reading frames (altORFs), increasing the size of the actual proteome. Top-down mass spectrometry-based proteomics allows the identification of intact proteins
Vivian Delcourt +12 more
doaj +1 more source
Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2 [PDF]
, 2017 We thank Zhen-Yuan Lin for help in the preparation of the AP-MS samples, and Cathy Collins for technical assistance. MDL is supported by a Sir Henry Wellcome Postdoctoral Fellowship (Wellcome Trust 096072), LEC is supported by a Canada Research Chair in ...
Bohovych, Iryna +7 more
core +4 more sources
FEBS Open Bio, EarlyView.Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen +6 more
wiley +1 more source
Green Analytical ChemistryWe present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform ...
Teresa Kwok +7 more
doaj +1 more source
Identification of calcium-binding proteins associated with the human sperm plasma membrane
Reproductive Biology and Endocrinology, 2010 Background The precise composition of the human sperm plasma membrane, the molecular interactions that define domain specific functions, and the regulation of membrane associated proteins during the capacitation process, still remain to be fully ...
Westbrook Anne +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Scientific ReportsThe aim of this study was to develop a rapid and easy-to-adapt protocol for recombinant production of Orthoflavivirus proteins. We introduced the new-generation pOpiE2 vector for virus-free transient transfection of Trichopluisa ni High Five insect cells
Luca Schelle +9 more
doaj +1 more source
Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications
FEBS Open Bio, EarlyView.This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley +1 more source
Structural host-virus interactome profiling of intact infected cells
Nature CommunicationsVirus-host protein-protein interactions (PPIs) are fundamental to viral infections, yet high-resolution identification of their structural and molecular determinants within the native context of intact infected cells has remained an unsolved challenge ...
Boris Bogdanow +7 more
doaj +1 more source
Prediction of protein-protein interactions using one-class classification methods and integrating diverse data [PDF]
, 2007 This research addresses the problem of prediction of protein-protein interactions (PPI) when integrating diverse kinds of biological information. This task has been commonly viewed as a binary classification problem (whether any two proteins do or do ...
Gilbert, D, Reyes, J A
core +3 more sources