Results 281 to 290 of about 199,615 (291)

Chapter 4. Liquid Chromatography-Mass Spectrometry of Intact Proteins

2011
In this chapter we address the use of liquid chromatography-mass spectrometry in the analysis of intact proteins. We cover multiple approaches and applications including quantitation, identification and the derivation of structural information. Because of the difficulty of intact protein analysis and the variety of approaches necessary for proteins of ...
Nicolas L. Young, Benjamin A. Garcia
openaire   +1 more source

Investigating Viral Proteins and Intact Viruses with Mass Spectrometry

2003
Mass spectrometry has become a fundamental technology for virology and an important tool in probing the structure and function of viruses. It has been used to identify viral capsid proteins, detect viral mutants, characterize post-translational modifications, and measure intact viruses. Mass-based analysis techniques including time resolved proteolysis,
Sunia A. Trauger, Teri Junker, Gary Siuzdak   +2 more
openaire   +1 more source

Enhanced-Fluidity Liquid Chromatography–Mass Spectrometry for Intact Protein Separation and Characterization

Analytical Chemistry, 2018
Recent advances in the analysis of proteins have increased the demand for more efficient techniques to separate intact proteins. Enhanced-fluidity liquid chromatography (EFLC) involves the addition of liquefied CO2 to conventional liquid mobile phases.
Yanhui Wang, Susan V. Olesik
openaire   +2 more sources

Enhancing the characterization of intact proteins by ultraviolet photodissociation mass spectrometry

2022
Access to high resolution mass spectrometers and high energy modes of activation such as electron- and photon-based modalities have enabled wider adoption of top-down methodologies, or strategies that allow the study of intact proteins. However, interpretation of MS/MS spectra of large proteins remains difficult owing to spectral congestion, charge ...
Dunham, Sean Duncan, 0000-0002-6214-7468
openaire   +2 more sources

Characterization of Clostridium species utilizing liquid chromatography/mass spectrometry of intact proteins

Journal of Microbiological Methods, 2009
A method for biomarker candidate discovery and strain level pathogen characterization using liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization is described. This method was applied to two pathogenic Clostridium species: C. difficile and C. perfringens.
Robert A, Everley, Tiffany M, Mott, Denise M, Toney, Timothy R, Croley   +3 more
openaire   +2 more sources

Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry

Journal of Proteome Research, 2019
Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein
Zheyi Liu, Ruimin Wang, Jing Liu, Ruixiang Sun, Fangjun Wang   +4 more
openaire   +2 more sources

Processing Complex Mixtures of Intact Proteins for Direct Analysis by Mass Spectrometry

Analytical Chemistry, 2002
For analysis of intact proteins by mass spectrometry (MS), a new twist to a two-dimensional approach to proteome fractionation employs an acid-labile detergent instead of sodium dodecyl sulfate during continuous-elution gel electrophoresis. Use of this acid-labile surfactant (ALS) facilitates subsequent reversed-phase liquid chromatography (RPLC) for a
Fanyu, Meng, Benjamin J, Cargile, Steven M, Patrie, Jeffrey R, Johnson, Shaun M, McLoughlin, Neil L, Kelleher   +5 more
openaire   +2 more sources

Identification of Unusual Bacterial Glycosylation by Tandem Mass Spectrometry Analyses of Intact Proteins

Analytical Chemistry, 2005
The characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote O-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. In this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the ...
Schirm, M., Schoenhofen, I., Logan, S., Waldron, K., Thibault, P.   +4 more
openaire   +2 more sources

Development of mass spectrometry strategies for intact protein applications

Herein the use of high resolution Orbitrap mass spectrometry for accurate mass assignment and structural characterization of proteins is described. An advanced fragmentation method, ultraviolet photodissociation (UVPD), allowed assignment of structural features by generating numerous diagnostic ions.
James, Virginia Kathryn, 0000-0002-1906-4018   +1 more
openaire   +1 more source

Structural Characterization of Intact G Protein γ Subunits by Mass Spectrometry

2002
Kevin L, Schey, Mark, Busman, Lana A, Cook, Heidi E, Hamm, John D, Hildebrandt   +4 more
openaire   +2 more sources