Results 81 to 90 of about 199,615 (291)

Gastrointestinal endogenous proteins as a source of bioactive peptides : Doctor of Philosophy in Nutritional Sciences at Riddet Institute, Massey University, Palmerston North, New Zealand [PDF]

, 2016
Gastrointestinal endogenous proteins (GEP) were investigated as a source of bioactive peptides. In silico and in vitro methods were used singly or in combination to study GEP-derived peptides after simulated digestion.
Acharya, Prasannalakshmi
core  

Forced Degradation Testing as Complementary Tool for Biosimilarity Assessment [PDF]

, 2019
Oxidation of monoclonal antibodies (mAbs) can impact their efficacy and may therefore represent critical quality attributes (CQA) that require evaluation.
Dyck, Yan Felix Karl, Jabs, Wolfgang, Joseph, Jan Felix, Parr, Maria Kristina, Rehm, Daniel, Sandig, Volker, Winkler, Karsten   +6 more
core   +1 more source

Detection of intact megaDalton protein assemblies of vanillyl‐alcohol oxidase by mass spectrometry [PDF]

Protein Science, 2000
AbstractWell‐resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%.
van Berkel, W.J.H., van den Heuvel, R.H.H., Versluis, C., Heck, A.   +3 more
openaire   +4 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Comparative Analysis of Glycoform Profiles Between Biosimilar and Originator Monoclonal Antibodies by Liquid Chromatography–Mass Spectrometry

Journal of the Turkish Chemical Society, Section A: Chemistry
Glycosylation is considered as a critical quality attribute for monoclonal antibodies (mAbs) and needs routine monitoring during production. This study aims to compare the glycoform profiles of biosimilar and four originator mAbs using ultra-performance ...
Ahmet Emin Atik
doaj   +1 more source

Characterization and quantification of intact 26S proteasome proteins by real-time measurement of intrinsic fluorescence prior to top-down mass spectrometry. [PDF]

PLoS ONE, 2013
Quantification of gas-phase intact protein ions by mass spectrometry (MS) is impeded by highly-variable ionization, ion transmission, and ion detection efficiencies.
Jason D Russell, Mark Scalf, Adam J Book, Daniel T Ladror, Richard D Vierstra, Lloyd M Smith, Joshua J Coon   +6 more
doaj   +1 more source

Structural Analysis of Monoclonal Antibodies with Top-down and Middle-down Electron Transfer Dissociation Mass Spectrometry: The First Decade

CHIMIA, 2022
Monoclonal antibodies (mAbs) are protein biotherapeutics with a proven efficacy toward fighting life-threatening diseases. Their exceptional healing potential drives the annual increase in the number of novel mAbs and other antibody-like molecules ...
Luca Fornelli, Daniel Ayoub, Kristina Srzentic, Konstantin Nagornov, Anton Kozhinov, Natalia Gasilova, Laure Menin, Alain Beck, Yury Tsybin   +8 more
doaj   +1 more source

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Characterisation of the semi-volatile component of Dissolved Organic Matter by Thermal Desorption – Proton Transfer Reaction – Mass Spectrometry [PDF]

, 2017
Proton Transfer Reaction – Mass Spectrometry (PTR-MS) is a sensitive, soft ionisation method suitable for qualitative and quantitative analysis of volatile and semi-volatile organic vapours. PTR-MS is used for various environmental applications including
Cook, Sarah, Gauci, Vincent, Holzinger, Rupert, Kent, Matthew, Materić, Dušan, Peacock, Michael, Rockman, Thomas   +6 more
core   +6 more sources

Fragmentation of multiply-charged intact protein ions using MALDI TOF-TOF mass spectrometry [PDF]

Journal of the American Society for Mass Spectrometry, 2008
Top down proteomics in a TOF-TOF instrument was further explored by examining the fragmentation of multiply charged precursors ions generated by matrix-assisted laser desorption ionization. Evaluation of sample preparation conditions allowed selection of solvent/matrix conditions and sample deposition methods to produce sufficiently abundant doubly and
Liu, Zhaoyang, Schey, Kevin L.
openaire   +2 more sources