Results 131 to 140 of about 2,528,554 (279)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

open access: yesFEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão   +3 more
wiley   +1 more source

Numerical method for solving scalar problem of monochromatic wave scattering from a screen with nonlinear transmission conditions

open access: yesИзвестия высших учебных заведений. Поволжский регион: Физико-математические науки
Background. The purpose of the study is to develop a numerical approach for solving the scalar diffraction problem on a flat screen with nonlinear transmission conditions. Material and methods.
V.O. Nesterov, A.A. Tsupak
doaj   +1 more source

Numerical solution of singular integro-differential Prandtl equation by the method of orthogonal polynomials

open access: yesЖурнал Белорусского государственного университета: Математика, информатика, 2019
The paper is constructed and proved computational scheme for a solution of singular Prandtl of integro-differential  equations with singular integral over the interval of the real axis, understood in the sense of the Cauchy principal value.
Galina A. Rasolko
doaj  

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Integral solutions of fractional evolution equations with nondense domain

open access: yesElectronic Journal of Differential Equations, 2017
In this article, we study the existence of integral solutions for two classes of fractional order evolution equations with nondensely defined linear operators.
Haibo Gu   +3 more
doaj  

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

open access: yesFEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro   +1 more
wiley   +1 more source

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