Results 91 to 100 of about 236,914 (218)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Discrepancies between declared and real practices of continuous renal replacement therapy for septic acute kidney injury in French intensive care units. [PDF]

Ann Intensive Care
Stephan L, Prevel R, Sazio C, Ilciukas-Miquel H, Auvet A, Bodot S, Chelha R, Coirier V, Fabre X, Garin A, Garraud J, Gisbert-Mora C, Grillet G, Hayon J, Labro G, Lambour A, Lancrey-Javal T, Le Basnier E, Mikulski M, Nicolas J, Olry K, Plouvier F, Seguin T, Tientcheu A, Valette M, Guisset O, Gruson D, Boyer A, Orieux A.   +28 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Prophylactic anti-seizure medication for post-traumatic seizures in Chinese PICUs: current practices and knowledge gaps. [PDF]

BMC Neurol
Ji J, Wang Q, Li Z, Fang B, Wang L, Qian S.   +5 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Clamping of the endotracheal tube to preserve the level of PEEP during disconnection from the ventilation circuit - a survey on routine practice. [PDF]

BMC Anesthesiol
Waydhas C, Brod T, Gliwitzky B, Herbstreit F, Menzen N, Pelz S.   +5 more
europepmc   +1 more source

[Microbiological survey in an Intensive Care Unit]

Le infezioni in medicina
An epidemiological survey on 417 sample from ICU patients, was carried out from January '97 to December 98 to verify the epidemiology of bacteria responsible for nosocomial infections and to monitor the susceptibility patterns to antimicrobial drugs during this period. Microbiological cultures were positive in 47.7% of the samples. P.
V., Cuniato, F., Bellitti, R., D'Isanto, A., De Sio, A., Marvaso, S., Esposito   +5 more
openaire   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Breastfeeding support in Italian Pediatric Intensive Care Units: a nationwide survey on protocols, practices, and challenges. [PDF]

Eur J Pediatr
Morniroli D, Daverio M, Davanzo R, Salvatori G, Agostoni C, Giannì ML, Amigoni A, Milani GP, MILPIC Study Group.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source