Results 181 to 190 of about 5,317,329 (303)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Perceptions on artificial intelligence among anaesthesia and intensive care professionals: An international survey on attitudes, expectations and needs. [PDF]

J Clin Monit Comput
Theilen R, Kramer T, Scharffenberg M, Jung IC, Zerlik M, Gama de Abreu M, Sepehr S, Koch T, Sedlmayr B, Huhle R, Wittenstein J.   +10 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Analgesia and sedation practice in children in intensive care in Australia and New Zealand: A survey. [PDF]

Crit Care Resusc
Holschier J, Veysey A, Masterson K, Long D, Erickson S, Cree M, Balit C, Butt W, Gelbart B.   +8 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Intensive care subspecialty in turkey: what do emergency medicine physicians think? [PDF]

Clinics (Sao Paulo)
Barut E, Giden R, Barut K, Yasak İH, Araç S.   +4 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

ICU Clinicians' View on Platelet Transfusion Thresholds for a Future Trial-Protocol for an International Survey. [PDF]

Acta Anaesthesiol Scand
Barakji JA, Anthon CT, Granholm A, Møller MH, Stensballe J, Perner A.   +5 more
europepmc   +1 more source

Geological Survey of Victoria. No. 2 [cartographic material]

Caption title.; Quarter sheet 2SW is a geological map centering on the town of Broadmeadows in the parish of Will Will Rook in the county of Bourke and showing some cadastral detail. Also includes part of the parish of Tullamarine. Quarter sheet 2NW is a
Geological Survey of Victoria.
core  

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source