Variation of rDNA Internal Transcribed Spacer Sequences in Rhizoctonia cerealis
Current Microbiology, 2017Fifty-four single protoplast isolates (SPIs) were regenerated from three Rhizoctonia cerealis strains. A total of 169 rDNA-ITS regions were cloned and sequenced from these 54 SPIs. Variations in the ITS1 and ITS2 regions that flank the 5.8S gene were found within clones from the same strain, as well as within clones from the same SPI.
Lei Ji +4 more
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Heterogeneity of the internal transcribed spacer 1 (ITS1) inTulipa (Liliaceae)
Plant Systematics and Evolution, 2000Heterogeneity of the internal transcribed spacer ITS1 of the rDNA within individuals ofTulipa gesneriana L.,T. kaufmanniana Regel, and their interspecific hybrids was analyzed by PCRRFLP, using the polymorphic restriction enzymesRsaI andHinfI, and by nucleotide sequence analysis.
Booy, G., van der Schoot, J., Vosman, B.
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Phylogeny of Arachis based on internal transcribed spacer sequences
Genetic Resources and Crop Evolution, 2010Phylogenetic analysis, based on nuclear rDNA internal transcribed spacers (ITS) of Arachis species, corroborated a broad sub-classification of the genus. Three clustering algorithms were used to generate dendrograms which showed that Arachis Sections Extranervosae, Heteranthae and Triseminata were most primitive, and Section Arachis was most advanced ...
Chuan Tang Wang +6 more
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Testing the utility of internally transcribed spacer sequences in coral phylogenetics
Molecular Ecology, 2004AbstractReefâbuilding corals often possess high levels of intraindividual and intraspecific ribosomal DNA (rDNA) variation that is largely polyphyletic between closely related species. Polyphyletic rDNA phylogenies coupled with high intraindividual rDNA variation have been taken as evidence of introgressive hybridization in corals.
Steven V, Vollmer, Stephen R, Palumbi
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Putative group I introns in the eukaryote nuclear internal transcribed spacers
Current Genetics, 2019Group I introns are mobile genetic elements that interrupt genes encoding proteins and RNAs. In the rRNA operon, introns can insert in the small subunit (SSU) and large subunit (LSU) of a wide variety of protists and various prokaryotes, but they were never found in the ITS region.
Daniele Corsaro, Danielle Venditti
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16S-23S rDNA internal transcribed spacer regions in four Proteus species
Journal of Microbiological Methods, 2009Proteus is a Gram-negative, facultative anaerobic bacterium. In this study, 813 Proteus 16S-23S rDNA internal transcribed spacer (ITS) sequences were determined from 46 Proteus strains, including 388 ITS from 22 P. mirabilis strains, 211 ITS from 12 P. vulgaris strains, 169 ITS from 10 P. penneri strains, and 45 ITS from 2 P. myxofaciens strains.
Boyang, Cao +6 more
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Nuclear rRNA transcript processing versus internal transcribed spacer secondary structure
Trends in Genetics, 2015rRNA is one of the few universal features of life, making it uniquely suited to assess phylogenetic relationships. The processing of the initial polycistronic rRNA transcript is also a conserved process, involving numerous cleavage events and the generation of secondary structures.
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Internal Transcribed Spacer (ITS)-PCR Identification of MRSA
2007Polymerase chain reaction (PCR) analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) followed by microchip gel electrophoresis was useful for identification of staphylococci and for strain delineation of Staphylococcus aureus. In the study presented in this chapter, 74 ITS patterns were demonstrated among 1,188 isolated colonies of S ...
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Restriction polymorphisms in the internal transcribed spacers and 5.8S rDNA ofSaccharomyces
Current Microbiology, 1992Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S.
F I, Molina, T, Inoue, S C, Jong
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Evolutionary divergence of ribosomal internal transcribed spacer 2 in lizards
Molecular Biology, 2006Internal transcribed spacers 1 and 2 (ITS1 and ITS2) are known to play an important role in rRNA maturation, yet the mechanism of their action is still not completely understood. Comparison of the ITS1 and ITS2 nucleotide sequences for various organisms reveals conserved regions, which are potentially involved in rRNA biogenesis, and yields new ...
A. S. Voronov +3 more
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