Results 261 to 270 of about 21,783 (297)

Intestinal organoids: roadmap to the clinic

American Journal of Physiology-Gastrointestinal and Liver Physiology, 2021
Recent advances in intestinal organoid research, along with encouraging preclinical proof-of-concept studies, have revealed significant therapeutic potential for induced pluripotent stem cell (iPSC)-derived organoids in the healing and replacement of severely injured or diseased bowel (Finkbeiner et al. Biol Open 4: 1462–1472, 2015; Kitano et al.
Magdalena Kasendra, Misty Troutt, Taylor Broda, W. Clark Bacon, Timothy C. Wang, Joyce C. Niland, Michael A. Helmrath   +6 more
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Small Intestinalization of Colon Using Ileum Organoids

Trends in Cell Biology, 2021
Given the mismatch in size and morphology of intestinal organoids with native intestine, their application in intestinal segment regeneration has yet to be achieved. By engrafting ileal organoids in epithelium-removed colon, Sugimoto et al. recently constructed transplantable colonic segments with functional ileal epithelium and a subepithelial ...
Jinjian, Huang, Ziyan, Xu, Jianan, Ren
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Intestinal organoids in infants and children

Pediatric Surgery International, 2019
Recent advances in culturing of intestinal stem cells and pluripotent stem cells have led to the development of intestinal organoids. These are self-organizing 3D structures, which recapitulate the characteristics and physiological features of in vivo intestinal epithelium.
Sinobol, Chusilp, Bo, Li, Dorothy, Lee, Carol, Lee, Paisarn, Vejchapipat, Agostino, Pierro   +5 more
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Generation and Manipulation of Rat Intestinal Organoids

Journal of Visualized Experiments, 2023
When using organoids to assess physiology and cell fate decisions, it is important to use a model that closely recapitulates in vivo contexts. Accordingly, patient-derived organoids are used for disease modeling, drug discovery, and personalized treatment screening.
Eleanor, Zagoren, Anderson K, Santos, Nadia A, Ameen, Kaelyn, Sumigray   +3 more
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Innervation of Human Intestinal Organoids

Journal of Visualized Experiments
The complexity of intestinal cytoarchitecture and function poses significant challenges for the creation of the bioengineered small intestine. Techniques for generating human intestinal organoids (HIOs) resembling human small intestine have been previously reported. HIOs contain epithelium and mesenchyme but lack other critical components of functional
Rachel C, Bordelon, Madushani, Herath, Allison L, Speer   +2 more
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Intestinal organoid technology and applications in probiotics

Critical Reviews in Food Science and Nutrition, 2023
The impacts of probiotics on maintaining the host's intestinal health have been extensively confirmed. Organoid technology revolutionizes intestinal health research by providing a unique platform to study the effects of probiotics. It overcomes challenges posed by animal models and 2D cell models in accurately simulating the in vivo environment.
Han Tan, Xiaoyong Chen, Chen Wang, Jiajia Song, Jiahui Xu, Yuhong Zhang, Huayi Suo   +6 more
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Generation of small intestinal organoids for experimental intestinal physiology

2020
Human intestinal organoids (HIOs) derived from pluripotent stem cells were first described almost a decade ago as a method to differentiate intestinal tissue containing both epithelium and supporting mesenchymal cells. The original protocol documents a directed differentiation approach to first induce definitive endoderm from pluripotent stem cells ...
Meghan, Capeling, Sha, Huang, Adriana, Mulero-Russe, Roberto, Cieza, Yu-Hwai, Tsai, Andres, Garcia, David R, Hill   +6 more
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Long-Term Culture of Intestinal Organoids

2018
The in vitro long-term expansion of primary intestinal epithelial cells has been hampered by the inability to maintain an immature stem cell population. Recent technical advances have led to the development of a novel in vitro culture system that can sustain intestinal stem cells (ISCs) using growth factors that mimic the intestinal microenvironment in
Seung Bum, Lee, Sung-Hoon, Han, Sunhoo, Park   +2 more
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Mouse Small Intestinal Organoid Cultures

The intestinal epithelium is a highly dynamic and self-renewing tissue that is crucial for maintaining gut homeostasis. It can be cultured in vitro from isolated crypts to form three-dimensional (3D) intestinal organoids. These organoids have the ability to proliferate and differentiate into various epithelial cell lineages, offering a more ...
Lei, Chen, Xiaoting, Xu
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Implantable synthetic organoid matrices for intestinal regeneration

Nature Cell Biology, 2017
Organoids are a powerful tool to study both physiological and disease processes. A completely synthetic matrix assembled from exchangeable modular parts has been developed and not only supports proliferation of human intestinal organoids derived from pluripotent embryonic stem cells, but also augments subsequent ad vivo implantation into injured murine
Jeffrey W, Brown, Jason C, Mills
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