Results 21 to 30 of about 21,783 (297)

Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids

Micromachines, 2022
In this study, we propose a microfluidic organoid-trapping device used to immobilize human intestinal organoids and apply fluidic stimuli to them. The proposed device has a microchannel with a trapping region with wall gaps between the channel walls and ...
Miki Matsumoto, Yuya Morimoto, Toshiro Sato, Shoji Takeuchi   +3 more
doaj   +1 more source

LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival. [PDF]

, 2018
Epithelial dysfunction and crypt destruction are defining features of inflammatory bowel disease (IBD). However, current IBD therapies targeting epithelial dysfunction are lacking.
Bayrer, James R, Escusa, Hazel S, Fletterick, Robert J, Ingraham, Holly A, Klein, Ophir D, Moore, David D, Nattiv, Roy, Suzawa, Miyuki, Wang, Hongtao   +8 more
core   +5 more sources

Bioengineering for intestinal organoid cultures [PDF]

Current Opinion in Biotechnology, 2017
Recent advances allow access to human cell-based intestinal organoids that recreate human physiology to levels not possible with conventional 2D cell cultures. Despite their huge potential, there are many challenges that remain. This review will cover recent bioengineering approaches to improve organoid maturation, scale up, reproducibility and ...
Ge-Ah, Kim, Jason R, Spence, Shuichi, Takayama   +2 more
openaire   +2 more sources

A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation

Frontiers in Cell and Developmental Biology, 2021
Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited.
Jenny Ostrop, Rosalie T. Zwiggelaar, Marianne Terndrup Pedersen, Marianne Terndrup Pedersen, François Gerbe, Korbinian Bösl, Håvard T. Lindholm, Alberto Díez-Sánchez, Naveen Parmar, Silke Radetzki, Jens Peter von Kries, Philippe Jay, Kim B. Jensen, Kim B. Jensen, Cheryl Arrowsmith, Cheryl Arrowsmith, Cheryl Arrowsmith, Menno J. Oudhoff   +17 more
doaj   +1 more source

Intestinal organoids for Cystic Fibrosis research [PDF]

Journal of Cystic Fibrosis, 2020
Significant progress has been made in the development of CFTR modulator therapy; however, current CFTR modulator therapies are only available for a minority of the CF-patient population. Additionally, heterogeneity in in vivo modulator response has been reported among individuals carrying homozygous F508del-CFTR, adding to the desire for an optimal ...
E. de Poel, J.W. Lefferts, J.M. Beekman
openaire   +3 more sources

Optimization of Vascularized Intestinal Organoid Model. [PDF]

Adv Healthc Mater
AbstractVasculature is crucial for maintaining organ homeostasis and metabolism. Although 3D organoids can mimic organ structures and patterns, they still lack vascular systems, limiting the recapitulation of physiological complexities. Although vascularization of organoids has been demonstrated by mixing Matrigel in fibrin, how the mixed gel niche ...
Wen Z, Orduno M, Liang Z, Gong X, Mak M.
europepmc   +3 more sources

A permeability assay for mouse intestinal organoids

STAR Protocols, 2022
Summary: Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of ...
Stijn Aaron den Daas, Ugo Soffientini, Shilpa Chokshi, Gautam Mehta   +3 more
doaj   +1 more source

Clinical applications and optimization of patient-derived organoids in intestinal diseases [PDF]

Organoid, 2022
Since the first successful establishment of organoids from adult intestinal stem cells, organoid technology has rapidly developed. With advances in normal organoid technology, intestinal disorders, such as colorectal tumors and inflammatory bowel disease,
Tae Il Kim
doaj   +1 more source

Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism. [PDF]

, 2017
Most differentiated cells convert glucose to pyruvate in the cytosol through glycolysis, followed by pyruvate oxidation in the mitochondria. These processes are linked by the mitochondrial pyruvate carrier (MPC), which is required for efficient ...
A Ootani, A Ralston, A Roostaee, AG Muntean, Aimee Flores, B Ohlstein, B-Z Chen, C Moolenbeek, C Stringari, C Yang, CA Micchelli, CA Micchelli, Carl S. Thummel, CCP Aires, Christian S. Earl, Claire Bensard, D Dutta, D Karolchik, Dean Y. Li, DK Bricker, Don Delker, Dona R. Wisidagama, E Berger, F Cao, H Jiang, H Li, H Li, H Tateno, Heather R. Christofk, Helong Zhao, I Martínez-Reyes, J Benavides, J Wang, J Wang, James E. Cox, Jared Rutter, Jason Tanner, JC Schell, Jeffrey Mohlman, JL Golob, John C. Schell, JS Wu, K Birsoy, K Ito, K Nishino, K Weber, Kristofor A. Olson, KT Pate, KW McCool, LB Sullivan, Lei Jiang, Lise K. Sorensen, LK Boroughs, LR Edmunds, LR Gray, M Uhlen, M Uhlen, MA Keller, Mary P. Bronner, MB Gerstein, MJ Dailey, MJ Rodríguez-Colman, N Barker, N Buchon, NM Vacanti, O Warburg, PA Vigueira, Peng Wei, Priyanka Kanth, R Camarda, Ralph J. DeBerardinis, RB Flavell, Ren Miao, RW Daniels, S Beyaz, S Herzig, S Simmini, T Bender, T Sato, T Sato, T Sato, T Simsek, T. Cameron Waller, William E. Lowry, X Yin, X-M Wang, Y-Y Fan   +86 more
core   +1 more source

Intestinal organoids for modelling intestinal development and disease [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2018
Gastrointestinal diseases are becoming increasingly prevalent in developed countries. Immortalized cells and animal models have delivered important but limited insight into the mechanisms that initiate and propagate these diseases. Human-specific models of intestinal development and disease are desperately needed that can recapitulate structure and ...
Kathryn L. Fair, Jennifer Colquhoun, Nicholas R. F. Hannan   +2 more
openaire   +2 more sources