Intracellular Metabolites in Marine Microorganisms during an Experiment Evaluating Microbial Mortality [PDF]
Metabolites, 2020 Metabolomics is a tool with immense potential for providing insight into the impact of biological processes on the environment. Here, we used metabolomics methods to characterize intracellular metabolites within marine microorganisms during a ...
Krista Longnecker +1 more
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Comprehensive metabolome analysis of intracellular metabolites in cultured cells [PDF]
STAR Protocols, 2022 Summary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites.
Ryuhei Kudo +4 more
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Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols [PDF]
Metabolites, 2017 Sample preparation is one of the most important steps in metabolome analysis. The challenges of determining microbial metabolome have been well discussed within the research community and many improvements have already been achieved in last decade.
Farhana R. Pinu +2 more
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Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors [PDF]
Biomolecules, 2023 Quantification of the concentration of particular cellular metabolites reports on the actual utilization of metabolic pathways in physiological and pathological conditions.
Alvaro Darío Ortega
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Comparison of Various Extraction Approaches for Optimized Preparation of Intracellular Metabolites from Human Mesenchymal Stem Cells and Fibroblasts for NMR-Based Study [PDF]
MetabolitesMetabolomics has proven to be a sensitive tool for monitoring biochemical processes in cell culture. It enables multi-analysis, clarifying the correlation between numerous metabolic pathways.
Slavomíra Nováková +10 more
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Theoretical Studies of Intracellular Concentration of Micro-organisms’ Metabolites [PDF]
Scientific Reports, 2017 With the rapid growth of micro-organism metabolic networks, acquiring the intracellular concentration of microorganisms’ metabolites accurately in large-batch is critical to the development of metabolic engineering and synthetic biology. Complementary to
Hai-Feng Yang +5 more
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Flux imbalance analysis and the sensitivity of cellular growth to changes in metabolite pools. [PDF]
PLoS Computational Biology, 2013 Stoichiometric models of metabolism, such as flux balance analysis (FBA), are classically applied to predicting steady state rates - or fluxes - of metabolic reactions in genome-scale metabolic networks. Here we revisit the central assumption of FBA, i.e.
Ed Reznik, Pankaj Mehta, Daniel Segrè
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Profiling of Intracellular Metabolites: An Approach to Understanding the Characteristic Physiology of Mycobacterium leprae. [PDF]
PLoS Neglected Tropical Diseases, 2016 Mycobacterium leprae is the causative agent of leprosy and also known to possess unique features such as inability to proliferate in vitro. Among the cellular components of M.
Yuji Miyamoto +5 more
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Intracellular pharmacokinetic study of zidovudine and its phosphorylated metabolites
Acta Pharmaceutica Sinica B, 2016 Zidovudine (AZT), the first drug approved by the US Food and Drug Administration for the treatment of human immunodeficiency virus (HIV) infection, is metabolized in the host cells to 5′-AZT triphosphate (AZT-TP) which inhibits HIV reverse transcriptase.
Lingli Mu +8 more
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Chemotaxonomic patterns in intracellular metabolites of marine microbial plankton
Frontiers in Marine Science, 2022 Most biological diversity on Earth is contained within microbial communities. In the ocean, these communities dominate processes related to carbon fixation and nutrient recycling.
Bryndan P. Durham +11 more
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