Results 181 to 190 of about 25,002 (226)
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Piezo-actuated mouse intracytoplasmic sperm injection (ICSI)
Nature Protocols, 2007The mouse is a genetically tractable model organism widely used to study mammalian development and disease. However, mouse metaphase II (mII) oocytes are exquisitely sensitive and intracytoplasmic sperm injection (ICSI) with conventional pipettes generally kills them.
Naoko, Yoshida, Anthony C F, Perry
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Intracytoplasmic sperm injection (ICSI) in small ruminants
Animal Reproduction Science, 2012Small ruminants are an important component of the global production systems of meat and wool, and their reproductive biology is well known. However, the incorporation of assisted reproduction techniques (ART) in the production systems of small ruminants is not as well developed as for other domestic species.
J, López-Saucedo +3 more
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Sheep oocyte activation after intracytoplasmic sperm injection (ICSI)
Reproduction, Fertility and Development, 1998The effect of calcium concentration on fertilization and activation was examined in oocytes injected in vitro with sperm. Oocytes were subjected to sperm injection, to sham injection or remained uninjected, and were then cultured for 19 h in bicarbonate-buffered synthetic oviduct fluid (BSOF) without calcium, or containing either calcium chloride or ...
M C, Gómez +3 more
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Intracytoplasmic sperm injection (ICSI): Risks reconsidered
Human Fertility, 2000The aim of this article is to address some of the questions raised by the introduction of new techniques to alleviate infertility, using intracytoplasmic sperm injection (ICSI) as an example. Although any new technique has the potential to cause unanticipated harm and it is probably true that no treatment is risk-free, current practice demands that ...
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Development and implementation of intracytoplasmic sperm injection (ICSI)
Reproduction, Fertility and Development, 1995The purpose of this paper is to elucidate the experimental steps that led to the development of intracytoplasmic sperm injection (ICSI) and its application in the human. ICSI has become the most successful micromanipulation procedure for treating male infertility.
G D, Palermo +4 more
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[The ICSI (Intracytoplasmic Sperm Injection)].
Minerva ginecologica, 1998ICSI (Intracytoplasmic Sperm Injection) is the latest known assisted reproduction technique (ART) and it already appears to be mature. In fact the analysis of the results presented by the researchers over the years has shown that the most specific indication for this ART is the sterility of the couple with serious male pathology, up to ejaculatory ...
N, Garcea, S, Campo, R, Garcea
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Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice
2014Over the years many well-described techniques for the introduction of transgene DNA into host organisms have been used, including pronuclear injection, in vitro fertilization-mediated transgenesis, transfection of ES and spermatogenic cells, nuclear transfer of somatic cell nuclei, and lentiviral vectors.
Pedro N, Moreira, Lluís, Montoliu
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Genetic considerations related to intracytoplasmic sperm injection (ICSI)
Fertility and Sterility, 2008This Committee Opinion outlines the genetic factors related to this procedure.
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Intracytoplasmic sperm injection (ICSI)
Fertility and Sterility, 2006Varsha Roy, C Ashraf
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Intracytoplasmic Sperm Injection (ICSI): Applications and Insights
2018When the oocyte (egg) and sperm combine in fertilization, one remarkable process is set in motion and another completed. The first is to initiate the establishment of totipotency, the faculty of a cell to give rise to an entire individual. The second is to produce a new and unique embryo genome comprising one meiotically rearranged nuclear contribution
Toru Suzuki, Anthony C. F. Perry
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