Results 61 to 70 of about 240,775 (234)
Advanced Functional Materials, EarlyView.Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley +12 more
wiley +1 more source
Phylogeny and evolution of chloroplast tRNAs in Adoxaceae
Ecology and Evolution, 2021 Chloroplasts are semiautonomous organelles found in photosynthetic plants. The major functions of chloroplasts include photosynthesis and carbon fixation, which are mainly regulated by its circular genomes. In the highly conserved chloroplast genome, the
Qiu‐Yi Zhong +6 more
doaj +1 more source
Switchable Supramolecular Adhesive by Tuning Interfacial Bonding and Modulus
Advanced Functional Materials, EarlyView.The supramolecular adhesive (HyDiP) shows reversible adhesion and recyclability. In the dehydrated state, it is dense, stiff (E ≈445 MPa), transparent, and provides strong bonding with adhesion strengths up to 4.65 MPa. In the hydrated state, it becomes porous, soft (E ≈0.11 MPa), and detaches easily, enabling sustainable high‐strength applications ...
Rumin Fu +10 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin +18 more
wiley +1 more source
Cell‐type specific analysis of translating RNAs in developing flowers reveals new levels of control
Molecular Systems Biology, 2010 Determining both the expression levels of mRNA and the regulation of its translation is important in understanding specialized cell functions. In this study, we describe both the expression profiles of cells within spatiotemporal domains of the ...
Yuling Jiao, Elliot M Meyerowitz
doaj +1 more source
Advanced Materials Interfaces, EarlyView.PEDOT:PSS‐coated 3DP microstructures are introduced to realize TENGs with enhanced mechanical compliance and high electrical performance. Among the investigated geometries, arch‐shaped microstructures exhibit superior fatigue resistance due to their spring‐like behavior, enabling efficient stress distribution, stable contact evolution, and sustained ...
Mariam E. Alhosani +9 more
wiley +1 more source
Introns as Gene Regulators: A Brick on the Accelerator
Frontiers in Genetics, 2019 A picture is beginning to emerge from a variety of organisms that for a subset of genes, the most important sequences that regulate expression are situated not in the promoter but rather are located within introns in the first kilobase of transcribed ...
Alan B. Rose
doaj +1 more source
In-Depth Analysis Reveals Production of Circular RNAs from Non-Coding Sequences
Cells, 2020 The sequencing of total RNA depleted for ribosomal sequences remains the method of choice for the study of circRNAs. Our objective was to characterize non-canonical circRNAs, namely not originating from back splicing and circRNA produced by non-coding ...
Annie Robic, Julie Demars, Christa Kühn
doaj +1 more source
Thermal Plasticity is Regulated by a Key MicroRNA During Range Expansion of an Invasive Fruit Fly
Advanced Science, EarlyView.Populations at the invasion front of Bactrocera dorsalis adapt through reduced phenotypic and gene expression plasticity, a phenomenon likely to be driven by genetic assimilation. We identify a key miRNA‐mediated regulatory axis, in which miR‐276b post‐transcriptionally represses thw, a conserved chitin‐binding gene critical to the cold‐tolerance ...
Yan Zhao +5 more
wiley +1 more source
Dynamics of the Mammalian Placental Metabolome in Placentogenesis and Embryonic Development
Advanced Science, EarlyView.This study identifies three metabolic stages (E8.5, E9.5–10.5, E11.5–14.5) and two transition periods (E8.5–9.5, E10.5–11.5) in mouse placental development. NAD(H) emerges as a key dynamic metabolite that enhances embryonic growth through accelerated segmentation and increased proliferation of mouse embryonic stem cell (mESC)‐induced presomitic ...
Gang Chen +11 more
wiley +1 more source