Results 171 to 180 of about 1,851 (202)

Biocatalytic properties of immobilized inulinase

Russian Journal of Bioorganic Chemistry, 2016
A heterogeneous biocatalyst based on inulinase immobilized on a nonionic sorbent of Stirosorb series was proposed. Thermal and acid inactivation of free and immobilized inulinase was examined and the corresponding inactivation constants were calculated.
I. V. Shkutina, O. F. Stoyanova, V. F. Selemenev   +2 more
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Inulinase from Chrysosporium pannorum

Journal of Fermentation Technology, 1988
Abstract A mold, Chrysosporium pannorum AHU 9700, isolated from soil, was found to produce a very active inulin-hydrolyzing enzyme. The inulinase activity of this strain was induced by inulin, but not by sucrose, glucose, or fructose. The highest inulinase activity, 115 units/ml, was obtained under the optimum conditions.
Rong Xiao, Masatoshi Tanida, Shoichi Takao   +2 more
openaire   +1 more source

Characterization of Sphingomonas sp. JB13 exo-inulinase: a novel detergent-, salt-, and protease-tolerant exo-inulinase

Extremophiles, 2015
A glycoside hydrolase family 32 exo-inulinase gene was cloned from Sphingomonas sp. JB13 and expressed in Escherichia coli BL21 (DE3). The purified recombinant enzyme (rInuAJB13) showed an apparently optimal activity at pH 5.5 and 55 °C and remained activity at 10-70 °C.
Junpei, Zhou, Mozhen, Peng, Rui, Zhang, Junjun, Li, Xianghua, Tang, Bo, Xu, Junmei, Ding, Yajie, Gao, Junrong, Ren, Zunxi, Huang   +9 more
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Biotechnological potential of microbial inulinases: Recent perspective

Critical Reviews in Food Science and Nutrition, 2016
Among microbial enzymes, inulinases or fructo-furanosylhydrolases have received considerable attention in the past decade, and as a result, a variety of applications based on enzymatic hydrolysis of inulin have been documented. Inulinases are employed for generation of fructose and inulo-oligosaccharides (IOS) in a single-step reaction with specificity.
Hemant Kumar, Rawat, Hemant, Soni, Helen, Treichel, Naveen, Kango   +3 more
openaire   +2 more sources

The cell wall-associated inulinase of Kluyveromyces fragilis

Antonie van Leeuwenhoek, 1984
The yeast Kluyveromyces fragilis (ATCC 12424) was grown on a 2% inulin-1% yeast extract medium for 36 h and subsequently fixed with 0.5% glutaraldehyde. The glutaraldehyde treatment did not affect the beta-fructofuranosidase (inulinase, EC 3.2.1.7) activity of the cells but it did make the cells resistant to chemical and physical treatments that ...
W E, Workman, D F, Day
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Screening for microorganisms that produce only endo-inulinase

Applied Microbiology and Biotechnology, 2001
Sixteen fungal strains reported in the literature as endo-inulinase producers and three bacterial strains, isolated from the dahlia rhizosphere, were analysed for endo-inulinase production. From four isolated strains (one fungus and three bacteria) the results were evaluated in terms of substrate consumption, cell growth and production of endo ...
R M, Gern, S A, Furlan, J L, Ninow, R, Jonas   +3 more
openaire   +2 more sources

The interaction of inulinase molecules with a chitosan matrix: UV-induced changes in the functional properties of immobilized inulinase

Biophysics, 2016
We have developed a technique for the immobilization of inulinase on chitosans with different molecular weights. The acid-soluble mid-molecular-weight (200 kDa) and high-molecular-weight (350 kDa) chitosans are shown to be promising matrices for inulinase adsorption.
M. G. Holyavka, V. G. Artyukhov, P. V. Seredin   +2 more
openaire   +1 more source

Molecular characterization and expression of microbial inulinase genes

Critical Reviews in Microbiology, 2012
Many genes encoding exo- and endo-inulinases from bacteria, yeasts and filamentous fungi have been cloned and characterized. All the inulinases have several conserved motifs, such as WMND(E)PNGL, RDP, EC(V)P, SVEVF, Q and FS(T), which play an important role in inulinase catalysis and substrate binding. However, the exo-inulinases produced by yeasts has
Guang-Lei, Liu, Zhe, Chi, Zhen-Ming, Chi
openaire   +2 more sources

Microbial Inulinases: Fermentation Process, Properties, and Applications

1983
Publisher Summary The microbial enzyme, inulinase, hydrolyzes the plant polymer inulin into practically pure fructose. Several methods have been applied to prepare stable crude Kluyveromyces fragilis inulinase preparations: acetone drying of cells, P 2 O 5 , drying, and cell autolysis yielded preparations which were stable at 0°C for at least one ...
E J, Vandamme, D G, Derycke
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Applications of Fungal Inulinases

2021
Ritumbhara Choukade, Naveen Kango
openaire   +1 more source