Results 171 to 180 of about 1,901 (203)

The cell wall-associated inulinase of Kluyveromyces fragilis

Antonie van Leeuwenhoek, 1984
The yeast Kluyveromyces fragilis (ATCC 12424) was grown on a 2% inulin-1% yeast extract medium for 36 h and subsequently fixed with 0.5% glutaraldehyde. The glutaraldehyde treatment did not affect the beta-fructofuranosidase (inulinase, EC 3.2.1.7) activity of the cells but it did make the cells resistant to chemical and physical treatments that ...
W E, Workman, D F, Day
openaire   +2 more sources

Screening for microorganisms that produce only endo-inulinase

Applied Microbiology and Biotechnology, 2001
Sixteen fungal strains reported in the literature as endo-inulinase producers and three bacterial strains, isolated from the dahlia rhizosphere, were analysed for endo-inulinase production. From four isolated strains (one fungus and three bacteria) the results were evaluated in terms of substrate consumption, cell growth and production of endo ...
R M, Gern, S A, Furlan, J L, Ninow, R, Jonas   +3 more
openaire   +2 more sources

Inulinase production by the yeast Kluyveromyces marxianus with the disrupted MIG1 gene and the over-expressed inulinase gene

Process Biochemistry, 2014
Kluyveromyces marxianus has been widely used in food industries and inulinase produced by the yeast has many applications. In order to greatly enhance inulinase production by the yeast, it is very important to both derepress glucose repression and overexpress the native inulinase gene. The MIG1 gene in the yeast K. marxianus KM-0 was disrupted.
Hai-Xiang Zhou, Fang-Hua Xin, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi   +4 more
openaire   +1 more source

The interaction of inulinase molecules with a chitosan matrix: UV-induced changes in the functional properties of immobilized inulinase

Biophysics, 2016
We have developed a technique for the immobilization of inulinase on chitosans with different molecular weights. The acid-soluble mid-molecular-weight (200 kDa) and high-molecular-weight (350 kDa) chitosans are shown to be promising matrices for inulinase adsorption.
M. G. Holyavka, V. G. Artyukhov, P. V. Seredin   +2 more
openaire   +1 more source

Molecular characterization and expression of microbial inulinase genes

Critical Reviews in Microbiology, 2012
Many genes encoding exo- and endo-inulinases from bacteria, yeasts and filamentous fungi have been cloned and characterized. All the inulinases have several conserved motifs, such as WMND(E)PNGL, RDP, EC(V)P, SVEVF, Q and FS(T), which play an important role in inulinase catalysis and substrate binding. However, the exo-inulinases produced by yeasts has
Guang-Lei, Liu, Zhe, Chi, Zhen-Ming, Chi
openaire   +2 more sources

Microbial Inulinases: Fermentation Process, Properties, and Applications

1983
Publisher Summary The microbial enzyme, inulinase, hydrolyzes the plant polymer inulin into practically pure fructose. Several methods have been applied to prepare stable crude Kluyveromyces fragilis inulinase preparations: acetone drying of cells, P 2 O 5 , drying, and cell autolysis yielded preparations which were stable at 0°C for at least one ...
E J, Vandamme, D G, Derycke
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Applications of Fungal Inulinases

2021
Ritumbhara Choukade, Naveen Kango
openaire   +1 more source

Inulinase

1991
Dietmar Schomburg, Margit Salzmann
openaire   +1 more source

Inulinases

2017
R.S. Singh, R.P. Singh
openaire   +1 more source

Inulinases

2006
Chandran Sandhya, Ashok Pandey
openaire   +1 more source